富含血小板血浆对小鼠触须毛乳头细胞活性和毛囊重建的影响  

Effect of PRP on the proliferation of dermal papilla cells and hair follicle regeneration in mice

作　　者：苗勇[1] 冯传波[1] 张志丹[1] 李泽华[1] 肖顺娥[1] 姜金豆[1] 胡志奇[1] 

机构地区：[1]南方医科大学南方医院整形美容外科,广州510515

出　　处：《中华整形外科杂志》2013年第2期131-135,共5页Chinese Journal of Plastic Surgery

基　　金：国家自然科学基金（31170949）;广东省教育部产学研结合项目（20118090400014）

摘　　要：目的探讨富含血小板血浆（platelet-rich plasma，PRP）对小鼠触须毛乳头细胞（dermal papilla cells，DPCs）活性和毛囊重建的影响。方法将采用2步离心法制备的PRP与采用显微解剖法获得的DPCs共培养，显微镜下观察细胞的形态变化，通过MTT法检测第4和第8代DPCs细胞活性。将新鲜分离的C57BL／6J小鼠背部表皮细胞与第4代的DPCs混合制成含有不同终浓度PRP的细胞悬液并移植至裸鼠体内，观察移植区域毛发形成的时间，4周后处死裸鼠，HE染色计算各组毛囊数量。结果通过2步离心法获得的PRP中含有的血小板数量约为全血的8．3倍，与未加PRP的对照组比较，终浓度为5％和10％PRP组不但可促进前6代DPCs的聚集性生长，还可促进前8代DPCs的增殖。在裸鼠体内实验中，10％PRP组长出毛发的最早时间和形成毛囊的数量分别为（18±1）d和（344±27）根，对照组为（20±1）d和（288±35）根，差异有统计学意义（P〈0．05）。结论PRP既能够增强DPCs的活性，也有助于毛囊的重建。Objective To investigate the effects of platelet-rich plasma （PRP） on the proliferation of dermal papilla cells （DPCs） and hair follicle regeneration. Methods PRP was prepared using the double-spin method and applied to DPCs. The proliferative effect of activated PRP on DPCs was measured using MTT assay. To understand the influence of activated PRP on the hair-inductive capacity of DPCs, freshly isolated epidermal cells and DPCs of passage 4 were resuspended, mixed with various concentrations of a PRP （0% , 5% or 10% ） and were then transferred to a grafting chamber, which was implanted onto the dorsal skin of nude mice. The chambers were removed 1 week after grafting and HF formation was monitored for 4 weeks; the graft site was harvested and processed for histological examination. Results Activated PRP increased the proliferation benefited the aggregative growth of DPCs. There are significant difference in the yield of hair follicles compared with 10% PRP （344± 27） with 0% PRP （288 ± 35） in the area of reconstituted skin （P 〈 0.05）. The areas treated with PRP demonstrated an increase in hair follicles density of 19.4%. Ten percent PRP （ 18 ± 1 ） d also can significantly shorten the time of hair formation, compared with 0% PRP （20 ± 1 ） d （P 〈 0.05）. Conclusions There is a considerable effect of PRP on the time of hair formation and the yield of hair follicles reconstitution.

关 键 词：血小板血浆 秃发 毛囊 

分 类 号：R622[医药卫生—整形外科]