介孔SiO_2的改性及对诺维信漆酶的交联固定化  

Modified Mesoporous Silica: Synthesis and Immobilization Function for Novozymes Laccase

作　　者：王磊[1] 陈诚[1] 田美娟[1] 杨宇翔[1] 罗健辉 丁彬[2] 王平美 

机构地区：[1]华东理工大学化学与分子工程学院,上海200237 [2]中国石油集团科学技术研究院,北京100083

出　　处：《无机化学学报》2013年第4期677-688,共12页Chinese Journal of Inorganic Chemistry

基　　金：中国石油天然气集团公司科学研究与技术开发项目《纳米级智能化学驱油剂研制》(2011A-1001)资助项目

摘　　要：采用十六烷基三甲基溴化铵(CTAB)为模板剂,四乙氧基硅烷(正硅酸乙酯,TEOS)为硅源,硝酸为催化剂来制备介孔SiO2,并采用后嫁接法对介孔SiO2进行氨基化改性。利用红外光谱(IR),X射线粉末衍射(XRD),差热-热重分析(DTA-TG),扫描电镜(SEM),元素分析,微电泳法及N2吸附-脱附方法对改性前后的产物进行表征。结果表明氨基已成功嫁接到介孔SiO2孔道中,改性后的介孔SiO2有序度有所下降,但仍为介孔材料;改性之后介孔材料的孔径、比表面积、孔体积均变小。等电点由原来的2.74变为4.75。本文还以氨基修饰的介孔SiO2为载体,通过交联剂戊二醛固定诺维信(Novozymes)工业级漆酶,并采用正交设计法对固定化条件进行了优化。研究表明漆酶经固定化后,其操作稳定性比游离酶高。Mesoporous silica was prepared by using hexadecyl template, tetraethoxysilane （tetraethylorthosilicate, TEOS） as the catalyst. The obtained sample was then modified via post modification was characterized by IR, DTA-TG, XRD, grafting trimethyl ammonium bromide （CTAB） as the silicon source and nitric acid as the hydrolysis method. The mesoporous silica before and after SEM, elemental analysis, micro-electrophoresis and N2 adsorption-desorption. The results show that the mesoporous silica has been modified by amino-groups, the material after modification still belongs to a family of mesoporous materials in spite of a slight decrease in the order degree. The surface area, pore size and pore volume become smaller, and the isoelectric point is decreased from 2.74 to 4.75 after the modification. The commercial Novozymes laccase is successfully immobilized in the inner surface of amino-functionalized mesoporous silica in this work by using glutaraldehyde as the crosslinking agent. The conditions of immobilization on amino-functionalized mesoporous silica were optimized by means of orthogonal experimental designs. The results show that the operational stability of the immobilized enzyme is significantly improved in comparison with the free enzyme.

关 键 词：介孔SIO2 嫁接法 改性 固定化 酶 

分 类 号：O613.72[理学—无机化学] Q814.2[理学—化学]