小鼠放射性肺损伤模型的建立与鉴定  

Establishment and identification of the mice model with radiation-induced lung injury

作　　者：陆忠华[1] 万美珍[2] 马燕[1] 刘芬菊[3] 

机构地区：[1]苏州大学附属常州肿瘤医院放疗科,江苏省213002 [2]苏州大学附属常州肿瘤医院病理科,江苏省213002 [3]苏州大学放射医学与公共卫生学院

出　　处：《江苏医药》2013年第7期761-763,F0003,共4页Jiangsu Medical Journal

基　　金：江苏省自然科学基金(BK2009102);常州市卫生局重大项目(ZD200818)

摘　　要：目的建立小鼠放射性肺损伤模型。方法 80只雌性C57BL/6小鼠随机分为四组:A、B和C三组每组24只小鼠,分别单次X线照射12、20和30Gy;D组8只小鼠作为空白对照。照射后1、3d和1、2、4、8、16、24周8个时点取出肺组织,行HE、VG染色,观察肺纤维化形成,RT-PCR测定肺组织转化生长因子(TGF)-β1的表达。结果 A、B、C组均出现典型放射性肺损伤及肺纤维化表现,随着照射剂量的增加,肺部病变加重,且肺部纤维化出现提前。与D组相比,A、B、C组8个时点肺组织TGF-β1含量均显著增高(P<0.01)。结论成功建立了放射性肺损伤小鼠动物模型。Objective To establish a mouse model with radiation-induced lung injury. Methods Eighty female mice were randomly divided into groups of A（12 Gy, 24 mice）, B（20 Gy, 24 mice）, C（30 Gy, 24 mice） and D（controls, 8 mice）. At eight time points of 1,3 d, and 1,2,4,8,16,24 weeks after irradiation, the lung tissues were observed by HE and VG staining and the expressions of transforming growth factor（TGF）-βl in the lungs were detected by RT-PCR Results The typical radiation-induced lung injuries and pulmonary fibrosis were seen in groups of A, B and C. As the increase of irradiation dosage, the lung lesions aggravated and pulmonary fibrosis appeared in advance. Compared with group D, the expressions of TGF-βl in groups of A,B and C were significantly higher at all time points（P〈0. 01）. Conclusion The mouse model of radiation-induced lung injury has been successfully established.

关 键 词：放射性肺损伤 动物模型 

分 类 号：R818[医药卫生—放射医学]