HtrA1在子痫前期孕妇血清、胎盘组织中的表达及意义  

The significance and expression of high temperature requirement A1(HtrA1) in serum and placenta of pre-eclampsia.

作　　者：李春芳[1] 苟文丽[1] 宗璐[1] 李扬[1] 赵丽娜[1] 

机构地区：[1]西安交通大学医学院第一附属医院妇产科,西安710061

出　　处：《现代妇产科进展》2013年第3期178-180,共3页Progress in Obstetrics and Gynecology

基　　金：国家自然科学基金资助项目(No:30872773)

摘　　要：目的:探讨HtrA1(high temperature requirement A1)在子痫前期孕妇血清、胎盘组织中的表达及意义。方法:HtrA1属分泌型蛋白,可采用酶联免疫吸附法(ELISA)测定同期住院孕妇的空腹血清HtrA1浓度,其中正常晚期妊娠30例、轻度子痫前期25例、重度子痫前期20例;同时应用免疫组化SABC法检测胎盘组织中HtrA1的表达。结果:正常晚期妊娠、轻度子痫前期及重度子痫前期孕妇血清HtrA1浓度分别为(160.63±32.52)pg/ml、(210.82±32.52)pg/ml及(233.68±38.61)pg/ml,组间差异显著(P<0.05);正常晚期妊娠、轻度子痫前期及重度子痫前期孕妇胎盘组织中HtrA的平均灰度值分别为156.03±5.07、149.89±2.69及140.05±4.96,组间差异显著(P<0.05)。结论:随着病情加重,孕妇血清及胎盘组织中HtrA1的表达显著增高。HtrA1与子痫前期的发生、发展有关。Objective： To investigate the expression and significance of high temperature requirement A1 （ HtrA1 ） in serum and placenta of pre：eclampsia. Methods： HtrA1 was secretory protein. 30 normal pregnant women （control group） and 45 women with hypertensive disorder-complicating pregnancy （study group ） （including 25 cases of mild preeclampsia and 20 cases of severe preeclampsia） were recruited at the same time. Measured the concentration of serum HtrAl by enzyme-linked immunosorbent assay （ELISA）, as while detected the expressions of HtrAl in placenta by immunohistochemieal SABC methods. Results：The concentration of serum HtrA1 was significantly higher in the study group than that in control group （P〈0.05）. The concentrations were （ 160. 63±32. 52 ） pg/ml, （ 210. 82±32. 52 ） pg/ml and （233.68±38.61）pg/ml, respectively. The same result was detected in the level of HtrAl expression （ the value of grey were 156.03±5. 07 ,149. 89±2.69 and 140.05±4.96） （P〈0.05）. Conclusion： With the development of pre-eclampsia, the expression of HtrA1 was increased. HtrAl may participate in the occurrence and development of pre-eclampsia.

关 键 词：子痫前期 HTRA1 丝氨酸蛋白酶类 妊娠 胎盘 血清 

分 类 号：R714.244[医药卫生—妇产科学]