反式玉米素对HaCaT细胞迁移及水通透性的影响  

The impact of trans-zeatin on the migration and water permeability of HaCaT cell

作　　者：蒋洁瑶 纪超 张美华 毕志刚[2] 

机构地区：[1]南京医科大学第一附属医院皮肤性病科,江苏南京210000 [2]南京医科大学附属明基医院,江苏南京210000

出　　处：《中国麻风皮肤病杂志》2013年第3期168-171,共4页China Journal of Leprosy and Skin Diseases

基　　金：国家自然科学基金项目(编号:810701297和81101188)

摘　　要：目的:评价反式玉米素(Trans-Zeatin,tZ)对HaCaT细胞的迁移和水通透性的影响。方法:RNA干扰实验;载有钙黄绿素的HaCaT细胞执行水的通透性测量。结果:反式玉米素缓解了UV导致的HaCaT细胞迁移能力的下降;并改善了HaCaT细胞由UV介导的水通透性的下降。结论:经Trans-Zeatin处理后可上调角蛋白细胞中小鼠水通道蛋白3(AQP3)的表达,促进HaCaT细胞的迁移和改善水的通透性。Objective： To investigate the effect of trans - zeatin on the migration and water permeability of HaCaT cells. Methods： RNA interference （RNAi） experiments; Cells were loaded with calcein and water permeability mea- surement was performed. Results： tZ attenuates UV- induced down- regulation of migration in cultured skin kerati- nocytes （HaCaT cells）, tZ attenuates UV- induced downregulation of water permeability in cultured skin keratino- cytes （HaCaT cells）. Conclusion： we found that tZ treatment, which upregulates AQP3 expression, enhances cell migration and water permeability in keratinocytes.

关 键 词：反式玉米素 水通道蛋白3 细胞迁移 水通透性 

分 类 号：R285[医药卫生—中药学]