Fluorescent detection of coenzyme A by analyte-induced aggregation of a cationic conjugated polymer  

作　　者：CHENG MingChao QIANG XiHuai DU ChiMin 

机构地区：[1]College of Resource & Environment, Shaanxi University of Science and Technology [2]Changzhou Sijie Biochemical Ltd

出　　处：《Chinese Science Bulletin》2013年第11期1256-1261,共6页

基　　金：supported by the Innovative Research Team of Shaanxi University of Science & Technology (TD12-04);the Doctoral Scientific Research Fund of Shaanxi University of Science & Technology (BJ09-15);National Natural Science Foundation of China (51103082)

摘　　要：A new cationic conjugated polymer was designed and synthesized to optically discriminate coenzyme A(CoA) among structurally similar biomolecules, ATP, ADP and AMP. The analyte-induced aggregation of the conjugated polymer by π-stacking between their main chains leads to the fluorescence quenching. Except for the similar adenosine and phosphate moieties as those in ATP, ADP and AMP, the CoA molecule also includes a long side chain that is favorable for hydrophobic interactions. Thus, CoA can form a complex with oppositely charged conjugated polymer by cooperative electrostatic and hydrophobic interactions, whereas ATP, ADP and AMP form the complexes with oppositely charged conjugated polymer mainly by electrostatic interactions. The increase of the ion strength of the assay solution screens the electrostatic attractions, and the remaining hydrophobic interactions dominate the formation of PFP-PTF/CoA complex. At this case, the quenching efficiency of PFP-PTF by CoA is much higher than that by ATP, ADP and AMP, which impart the PFP-PTF to sense CoA from these interferencing species.A new cationic conjugated polymer was designed and synthesized to optically discriminate coenzyme A（CoA） among structurally similar biomolecules, ATP, ADP and AMP. The analyte-induced aggregation of the conjugated polymer by n-stacking between their main chains leads to the fluorescence quenching. Except for the similar adenosine and phosphate moieties as those in ATP, ADP and AMP, the CoA molecule also includes a long side chain that is favorable for hydrophobic interactions. Thus, CoA can form a complex with oppositely charged conjugated polymer by cooperative electrostatic and hydrophobic interactions, whereas ATP, ADP and AMP form the complexes with oppositely charged conjugated polymer mainly by electrostatic interactions. The increase of the ion strength of the assay solution screens the electrostatic attractions, and the remaining hydrophobic interactions dominate the formation of PFP-PTF/CoA complex. At this case, the quenching efficiency of PFP-PTF by CoA is much higher than that by ATP, ADP and AMP, which impart the PFP-PTF to sense CoA from these interferencing species.

关 键 词：共轭聚合物 荧光猝灭 辅酶A 阳离子 检测分析 聚集 诱导 疏水相互作用 

分 类 号：Q55[生物学—生物化学]