重组人促红细胞生成素对大鼠脊髓损伤后STAT5表达的影响  

The effect of recombinant human erythropoietin on the expression of STAT5 in the acute spinal cord injury rat

作　　者：邵博[1] 富长海[1] 李洪鹏[1] 

机构地区：[1]中国医科大学基础医学院解剖学教研室,沈阳110001

出　　处：《解剖科学进展》2013年第2期114-116,共3页Progress of Anatomical Sciences

基　　金：国家自然科学基金资助项目(No.81171248);沈阳市科技计划项目(No.111400TGF-β1/)

摘　　要：目的研究重组人促红细胞生成素(recombinant human erythropoietin,rHuEPO)对急性脊髓损伤大鼠信号传导子及转录激活子STAT5(signal transducer and activator of transcription5)表达的影响。方法成年健康SD大鼠72只,雌雄不限,切除T8、T9椎板,参照Nystrom's压迫方法制作大鼠脊髓压迫损伤模型,取T10节段脊髓组织,随机分为假手术组8只、急性脊髓损伤32只、rHuEPO处理组32只。利用免疫组化方法和Western blot方法检测各组大鼠STAT5的表达。结果免疫组化和Western blot方法结果发现,假手术组大鼠脊髓有大量的STAT5阳性表达,急性脊髓损伤组大鼠的STAT5阳性表达明显降低,3d时降至最低;与脊髓损伤组相比,相同时间点的经重组人促红细胞生成素处理的大鼠脊髓STAT5阳性表达明显升高(P<0.05)。结论 rHuEPO能够上调急性脊髓损伤大鼠STAT5的表达。Objective To study the effect of recombinant human erythropoietin（rHuEPO） on the expression of signal transducer and activator of transcription 5（STAT5） in the acute spinal cord injury rat. Methods 72 healthy adult SD rats were chose and randomly divided into the sham group, the acute spinal cord injury group and the recombinant human erythropoietin group. The spinal cord injury was established by Nystrom＇ s way. The expression of STAT5 in T10 segment was detected by immunochemistry and Western blot methods. Results Plenty of the positive STAT5 product in T10 segment of the spinal cord was found in sham group, and the positive expression level of STAT5 decreased significantly in spinal cord injury group than in sham control, lowest at 3 days after the injury, abut increassed significantly in rHuEPO treated group than in the acute spinal cord injury group by immunochemistry and Western blot methods. Conclusion I＇MuEPO can upregulate the expression of STAT5 in acute spinal cord injury rats.

关 键 词：脊髓损伤 重组人促红细胞生成素 STAT5 大鼠 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]