插入序列共同区在多种临床菌株间的分布及其与超广谱β-内酰胺酶基因bla_(PER-1)的关系研究  

Distribution of ISCR1in clinical strains and its relationship with bla_(PER-1) gene

作　　者：陈霞[1] 李桂喜[2] 禹惠兰[1] 袁敏[1] 侯小平[2] 李娟[1] 

机构地区：[1]中国疾病预防控制中心传染病预防控制所,细菌耐药室,传染病预防控制国家重点实验室,北京102206 [2]中国人民解放军第二五一医院检验科

出　　处：《疾病监测》2013年第3期178-183,共6页Disease Surveillance

基　　金：传染病预防控制国家重点实验室项目(No.2012SKLID205)~~

摘　　要：目的研究临床来源的多种类细菌中插入序列共同区(insertion sequence common regions,ISCRs)及超广谱β-内酰胺酶基因blaPER-1的分布情况,分析可移动元件ISCR1与细菌多药耐药表型及blaPER-1基因散播的关系。方法收集2011-2012年河北省某医院临床独立菌株,应用梅里埃VITEK2 COMPACT全自动微生物生化鉴定系统结合16S rDNA序列分析进行种属鉴定,利用普通聚合酶链反应(polymerase chain reaction,PCR)方法进行ISCR1、blaPER-1和ISCR1-blaPER-1间区的扩增;分析ISCR1、blaPER-1种属分布状况及ISCR1与菌株耐药性的关系。结果共得到17种(属)314株临床独立菌株,其中ISCR1携带率为28.7%(90/314),在不动杆菌和肠杆菌科的多种细菌中携带较多;携带ISCR1的菌株中,96.7%(87/90)为多药耐药菌株,93.3%(84/90)耐受9类及以上抗菌药物;blaPER-1基因携带率为12.4%(39/314),在7种细菌检出,不动杆菌属细菌中检出最多(31株);21株不动杆菌属细菌和1株普通变形杆菌存在ISCR1-blaPER-1结构。结论 ISCR1在多种细菌中广泛分布,细菌多药耐药表型和其携带有一定关系;blaPER-1基因在多种细菌中散播,ISCR1的基因捕获和移动机制可能造成了blaPER-1基因在不动杆菌属细菌中及在不同种细菌间的散播。Objective To understand the distribution of blaeER_lgene and ISCR1 in clinical isolates of different species and the relationships between ISCR1 and multidrug resistance, and between ISCR1 and blapER-1 gene. Methods Clinical isolates were collected from a hospital in Hebei province during 2011-2012. The species of the isolates were identified by VITEK- 2 COMPACT automatic microbial identification system and 16S rDNA sequence analysis. The distributions of ISCR1, blapER-1 gene and ISCRI-blapER-1 were detected by PCR. Results A total of 314 clinical isolates of different species were collected. ISCR1 and blapER_1 gene were detected in 28.7% and 12.4% of the isolates, respectively. ISCR]-blapER_1 sequence structure was detected in 1 Proteus vuigaris strain and 21 Acinetobacter spp. strains. Up to 96. 7% of ISCR1 contained strains were multidrug resistant, and 93.3% of them were resistant to all 9 antibiotics. Conclusion ISCR1 was distributed in various species of clinical isolates widely, which was related with mnltidrug resistance in clinical bacteria, blapER-1 gene was distributed in various species of clinical isolates. ISCR1 might facilitate the spread of blapER_1 gene among Acinetobacter spp. and other bacteria species.

关 键 词：ISCR1 blaPER-1基因 超广谱Β-内酰胺酶 多药耐药 

分 类 号：R446.5[医药卫生—诊断学]