萧山区2007年-2010年宋内志贺菌的病原分子生物学特征  

The pathogen molecular biologic characterization of Shigella sonnei strains in Xiaoshan during 2007-2010

作　　者：丁水军[1] 陈棋炯[1] 汪皓秋[2] 孙永祥[1] 傅丹青[1] 

机构地区：[1]杭州市萧山区疾病预防控制中心,杭州311201 [2]杭州市疾病预防控制中心,杭州310021

出　　处：《中国卫生检验杂志》2013年第3期551-553,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:了解2007年-2010年萧山区分离的宋内志贺菌的病原分子生物学特征,并分析其流行规律。方法:对130株宋内志贺菌进行生化反应和血清分型鉴定,利用PCR方法检测侵袭性质粒抗原H(ipaH)基因。使用脉冲场凝胶电泳(PFGE)进行同源性分型分析,并使用BioNumerics进行聚类分析以确定菌株间的亲缘关系。结果:130株菌株均为宋内志贺菌,均具有ipaH基因。PFGE分型将菌株分成90个型别,相似性72.4%～97.9%,主要分为A、B、C三个克隆群。结论:PFGE分型结果表明2007年-2010年萧山区的宋内志贺菌来源具有多样性,A、B、C三个克隆群的菌株占据主导地位,应引起重视。Objective： To explore the pathogen molecular biologic characterization of Shigella sonnei （ S. sonnei） isolates in Xiaoshan during 2007 -2010, and analyze the epidemic rules. Methods： One hundred and thirty S. sonnei strains were identified by biochemical tests and serotyping. Among these isolates, invasive plasmid antigen H gene （ipaH） was examined by PCR. Then the homology analysis was performed with Pulse -field gel electrophoresis （PFGE） and clustering of patterns was analyzed with the BioNumerics software. Results： All isolates were serotyped as S. sonnei and were positive for ipaH gene. A total of 90 PFGE patterns were identified with a similarity coefficient from 72.4% to 97.9%. They were mostly belonging to A, B and C patterns group. Conclusion： There is high level prevalence of S. sonnei in Xiaoshan and it has complicated source of diversity, most belong to A, B and C group and should be brought to the forefront.

关 键 词：宋内志贺菌 IPAH基因 脉冲场凝胶电泳 分型 

分 类 号：R378.25[医药卫生—病原生物学]