江苏省肠出血性大肠杆菌O157:H7PFGE分子分型研究  

Study on molecular subtyping of Enterohemorrhagic Escherichia coli O157:H7 in Jiangsu

作　　者：顾玲[1] 周璐[1] 董晨[1] 崔志刚[2] 谈忠鸣[1] 张艺飓[1] 

机构地区：[1]江苏省疾病预防控制中心,南京210009 [2]中国疾病预防控制中心传染病预防控制所,北京102206

出　　处：《中国卫生检验杂志》2013年第3期565-568,共4页Chinese Journal of Health Laboratory Technology

摘　　要：目的:对我省历年分离的不同地区和来源EHEC O157:H7菌株之间的同源性进行分析。方法:用限制性内切酶XbaI,对分离菌株进行PFGE分型,并使用BioNumerics Version 4.0软件(Dice系数和UPGMA法)进行聚类分析。结果:74株O157:H7分离株可分为39个型,同一年份同一地区不同来源菌株之间有相同的XbaI酶切带型,同一年份不同地区分离菌株之间有相同酶切带型,不同年份部分菌株之间酶切带型不可区分。结论:我省宿主动物携带多个O157:H7克隆,来自同一个克隆的O157:H7已在较广泛的区域内传播,某些在1999年暴发流行的克隆长期稳定存在于我省宿主动物中,并传播给人类。Objective ：To analyze the association of isolates of Enterohemorrhagic Escherichia coli O157：H7 from different sources in Jiangsu during 1999 -2009. Methods： Pulse -field gel electrophoresis （PFGE） using restriction enzyme Xbal was employed for the molecular subtyping of Enterohemorrhagic Escherichia coli O157 ： H7 isolated from 1999 to 2009, and PFGE patterns were analyzed by BioNumerics Version 4.0 software to perform cluster analy- sis. Pattern profiles were compared by utilizing Dice coefficient and UPGMA （unweighted pair group method with arithmetic averages）. Results： PFGE subtyping after XbaI DNA digestion produced a total of 39 distinct restriction endonuclease digestion profiles among the 74 strains. Some isolates from different sources at the same area at the same time had indistinguishable XbaI patterns. Part of isolates from distinct areas at the same time shared the identi- cal XbaI patterns. Some isolates from different time also had indistinguishable XbaI patterns. Conclusion： Many clones of Enterohemorrhagic Escherichia coli O157 ： H7 were shed by the feces of animals in Jiangsu and the strains from the identical clones had been spread in wide range in Jiangsu. Some of clones occurred in outbreak in 1999 had stably exited among animals for long time and disseminated to human.

关 键 词：肠出血性大肠杆菌O157 H7 脉冲场凝胶电泳(PFGE) 分子分型 聚类分析 

分 类 号：R378.21[医药卫生—病原生物学]