PMMA义齿基托材料中甲基丙烯酸甲酯的高效液相色谱测定及其溶出特性研究  

Determination of residual methyl methacrylate in the PMMA denture base materials by high performance liquid chromatography and its dissolving characteristic

作　　者：汤健彬[1] 吕昌银[1] 李芳[2] 管芳[1] 邵美玲[1] 孙强[1] 

机构地区：[1]南华大学公共卫生学院卫生理化检验系,湖南衡阳421001 [2]湖南省衡阳市疾病预防控制中心理化检验科,湖南衡阳421001

出　　处：《中国卫生检验杂志》2013年第3期572-575,共4页Chinese Journal of Health Laboratory Technology

基　　金：湖南省自然科学基金委员会与衡阳市人民政府自然科学联合基金资助(11JJ8008号)

摘　　要：目的:建立聚甲基丙烯酸甲酯(PMMA)义齿基托材料中残留甲基丙烯酸甲酯(MMA)的高效液相色谱测定方法,并分析它在3种提取液中的溶出特性。方法:提取液经Agilent TC-C18色谱柱分离,用乙腈-水(70+30)溶液洗脱,在波长210 nm处进行检测。结果:MMA浓度为0.05μg/ml～100μg/ml时,MMA的峰面积与浓度之间呈现良好的线性关系,检出限为0.03μg/ml,回收率为99.5%～102%。结论:该方法灵敏度高,准确性好,MMA在52°白酒中的溶出量很大,在生理盐水中的溶出量较小,在水中的溶出量很小(≤0.44 mg/g)。Objective：To develop a high performance liquid chromatographic method for the determination of residual methyl methacrylate （MMA） in PMMA denture base materials, and analyse its dissolving characteristics in the three extract solutions respectively. Methods ： Agilent TC - C18 column was used target analytes separation and aeetonitrile- H2O mixture （70 ＋ 30） was used as mobile phase in the separation. MMA was determined at 210 nm. Results： Linear relationship between values of peak area and mass concentration of MMA was obtained in the range of 0.05 μg/ml - 100 μg/ml with detection limit of 0.03 ixg/ml. Values of recovery ranged from 99.5% to 102%. Conclusion： The method was sensitive and accurate. The dissolving out amount of MMA was very large in the 52° wine, lesser in the physiological saline and minimum（ ≤0.44 mg/g） in the water

关 键 词：甲基丙烯酸甲酯 高效液相色谱法 义齿基托材料 溶出 

分 类 号：O657.72[理学—分析化学]