加压水解Sn^(2+)催化显色快速测定牛奶蛋白质  

Fast spectrophotometric determination of protein in milk under Sn^(2+) catalysis after pressurized hydrolysis

作　　者：金良正 曹丽军[1] 樊宏[2] 

机构地区：[1]浙江省金华市疾病预防控制中心,浙江金华321000 [2]浙江省金华市婺城区疾病预防控制中心,浙江金华321000

出　　处：《中国卫生检验杂志》2013年第3期595-596,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:建立牛奶蛋白质的快速光度分析方法。方法:将牛奶与1+1硫酸混合置于电压力锅内,加热加压水解40 min,水解产生的氨基酸在Sn2+催化下,与茚三酮反应显色后,分光光度法测定。结果:牛奶中蛋白质含量在3 mg～18 mg之间时与吸光度成线性,r=0.9992,最低检测量0.5 mg,方法回收率98.3%～102%,相对标准偏差2.45%～3.98%。结论:建立的方法适用于牛奶中蛋白质的快速测定。Objective： To establish a method for fast determination of protein in milk by spectrophotometry. Methods： After the milk was hydrolysed with H2 SO4 （ 1 ＋ 1 ） under heated and pressurized condition for 40 min, the amino acids decomposed from protein reacted with ninhydrin to form colored products under catalysis of Sn^2＋ and the colored products were determined by spectrophotometry. Results： The linear range of the method was 3 mg -18 mg （r = 0. 9992） with the detection limit of 0.5 mg and the recoveries of 98.3 % -102%, and the RSDs were 2.45% 3.98%. Conclusion ： The developed method was suitable for the fast determination of protein in milk.

关 键 词：牛奶 蛋白质 压力水解 Sn2+催化 

分 类 号：O657.31[理学—分析化学]