非离子表面活性剂OP增敏乙酰丙酮光度法测定海产品中的痕量甲醛  

The sensitized reaction of non-ionic surfactant to acetylacetone spectrophotometry in the determination of trace formaldehyde in marine products

作　　者：任淑华[1] 郭振良[1] 

机构地区：[1]鲁东大学化学与材料科学学院,山东烟台264025

出　　处：《中国卫生检验杂志》2013年第3期616-618,621,共4页Chinese Journal of Health Laboratory Technology

摘　　要：目的:建立快速、准确、灵敏测定海产品中甲醛的新方法。方法:在沸水浴中,痕量甲醛与乙酰丙酮能发生微弱的显色反应。实验发现,非离子表面活性剂OP对上述反应有较强的增敏作用,由此建立了一种测定痕量甲醛的新方法。结果:在测定条件下,溶液的最大吸收波长在412 nm,方法的检出限为0.11μg/ml,相关系数为0.9993,九次空白实验的标准偏差为S=0.00365,相对标准偏差为RSD为0.376%,回收率在100%～105%。结论:方法简单快速、选择性好,用于测定海产品中的甲醛时,测得结果与非增敏乙酰丙酮光度法及HPLC法的结果一致。Objective： To develop a simple, exact and sensitive method for determination of trace formaldehyde in marine products. Methods： In boiling water bath, aeetylacetone and formaldehyde could have a feeble colour reaction. The experiment was found that non - ionic surfactant OP could sensitize the colour reaction. Results： The maximum absorption wavelength was 412 nm. The detection limit was 0.11 μg/ml. The correlation coefficient was 0. 9993. The standard deviation and the relative standard deviation for nine blank were 0. 00365 and 0.376%. The recoveries were in the range of 100% to 105%. Conclusion： The results obtained in the application of this method to the analysis of marine products were in consistency with those obtained by the acetylacetone spectrophotometry and the HPLC method.

关 键 词：表面活性剂OP 甲醛 乙酰丙酮分光光度法 增敏 

分 类 号：O657.32[理学—分析化学]