人类Cx43基因的生物信息学分析及其法医学研究意义  

Bioinformatics analysis and forensic medicine significance of Cx43 gene

作　　者：王杰[1] 戴佳琳[1] 胡旭初[2] 汪元河[1] 黄映康[1] 黄江[1] 

机构地区：[1]贵阳医学院法医学教研室,贵阳550004 [2]中山大学北校区寄生虫学教研室,广州510080

出　　处：《中国卫生检验杂志》2013年第3期667-669,共3页Chinese Journal of Health Laboratory Technology

基　　金：贵州省科技攻关项目[黔科合NY字(2010)3090]

摘　　要：目的:生物信息学方法分析和预测人类Cx43(connexin 43)全长基因及其编码蛋白的结构和功能特征,探索其在法医学中的研究前景。方法:利用生物信息网站如美国国家生物技术信息中心(NCBI)和瑞士的生物信息学研究所的蛋白分析专家系统(ExPASY)中相关的基因和蛋白的序列和结构信息以及各种分析工具,并结合其它大型生物信息学分析软件包,如Vector NTI suite,分析该基因的结构并预测其编码的蛋白质的结构和功能特征等。结果:该基因全长1828 bp,编码382个氨基酸,含有四个跨膜区,含有多个磷酸化位点,其理论分子量为42960.3Da,具有较稳定的理化性质。结论:应用生物信息方法分析人类Cx43基因的功能和结构,对其法医病理学鉴定具有重要意义。Objective： To analyze and predict the structure and characteristics of connexin 43 gene by bioinformatics, in order to guide and discuss the experimental research in the field of the forensic. Methods： By using the analyzing tools of bioinformatics in bioinformatics webs sites such as NCBI, ExPaSy and combining other complicated bioinformatics software package such as Vector NTI suite to recognize connexin 43 gene and analyze its structure and coding region sequence, so as to analyze and predict structure and characteristics of the gene. Results： The full gene is 1828 bp in length, coding 382 amino acids. The protein had stable physical chemical characteristics, the molecular weight of adenylate kinase gene is predicted to be 42960.3Da. It had four transmembrane region and more phosphorylation sites. Conclusion： Connexin 43 gene was screened from the GenBank of homo. Bioinformatics method for analysis of Cx43 gene had vital significance in forensic pathology identification.

关 键 词：人类 CX43基因 生物信息学 法医学 

分 类 号：D919[医药卫生—法医学]