临床分离志贺菌中CTX-M-9型超广谱β-内酰胺酶基因分析  

Gene analysis for the CTX-M-9 group extended spectrum beta-lactamases in clinical isolates of Shigella

作　　者：苗元[1] 刘静[1] 王利萍[1] 崔京辉[1] 王永全[1] 吴本和[1] 靳博[1] 苗智慧[2] 

机构地区：[1]北京市西城区疾病预防控制中心,北京100120 [2]河北省疾病预防控制中心,石家庄050021

出　　处：《中国卫生检验杂志》2013年第3期670-672,共3页Chinese Journal of Health Laboratory Technology

基　　金：北京市西城区卫生局青年科技人才(科技新星)培养项目(2012W1113)

摘　　要：目的:了解本地区临床分离志贺菌中CTX-M-9型超广谱β-内酰胺酶(ESBLs)分布,分析CTX-M-9型基因的种类、数量以及多态性。方法:收集2008年-2011年本地区临床分离非重复的志贺菌94株,聚合酶链反应(PCR)检测CTX-M-9型ESBLs基因,对PCR阳性产物进行测序,测序结果在GenBank上比对确定基因亚型,并通过DNAman软件对核酸序列进行分析,确定SNPs位点。结果:94株志贺菌中,17株携带CTX-M-9型ESBLs基因,阳性率18.09%,并在福氏志贺菌、宋内志贺菌和鲍氏志贺菌中均有检出。对DNA序列进行比对、分析,均为CTX-M-14亚型,未发现SNPs位点。结论:本地区志贺菌中CTX-M-9型ESBLs以CTX-M-14亚型为主,基因结构稳定,未发生变异,并可广泛在志贺菌各血清型中存在。Objective：To understand the genotype distribution of CTX - M -9 group extended spectrum beta - lactamases （ESBLs） in clinical isolates of ShigeUa in Xicheng District of Beijing, and to analyze the species, quantity and polymorphism of CTX - M - 9 group gene. Methods： Ninety - four strains of non - reduplicative clinical isolates of Shigella were colleted from 2008 to 2011 in Xicheng District of Beijing,the CTX - M -9 group ESBLs gene was detected by polymerase chain reaction（ PCR）, then sequencing for PCR positive productions were performed, the sequencing results were compared in GenBank to determine gene subtypes, meanwhile the nucleotide sequence was analyzed by means of DNAman software to determine SNPs site. Results： Among 94 strains of Shigella, Seventeen strains（ 18.09% ） carried CTX - M - 9 group ESBLs gene, furthermore ,which was detected in ShigeUaflexneri ,Shigella sonnei and Shigella boydii. The results of DNA sequence analysis showed that CTX - M - 9 group ESBLs were all CTX - M - 14 subtype, and no SNPs site was found. Conclusion： The CTX - M - 9 type ESBLs in Xicheng Dis- trict of Beijing are mainly CTX -M -14 subtype, and the gene structure is stable,without gene mutation ,which exist widely in different serum types of Shigella.

关 键 词：志贺菌 超广谱Β-内酰胺酶 CTX—M-9型 耐药基因 

分 类 号：R378.25[医药卫生—病原生物学]