细胞芯片法与流式细胞法检测淋巴细胞亚群的比较  

Comparison of lymphocyte subpopulations detection with cell chip and flow cytometry

作　　者：彭赛蛟[1] 罗云[1] 闻树群[2] 张瑾[3] 

机构地区：[1]浙江省台州市中医院检验科,浙江台州318000 [2]浙江大学医学院附属二院,杭州310009 [3]浙江省台州市立医院检验科,浙江台州318000

出　　处：《中国卫生检验杂志》2013年第3期712-714,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:比较细胞芯片法与流式细胞法测定淋巴细胞亚群(CD3+,CD4+和CD8+)计数的准确度。方法:分别采用细胞芯片法和流式细胞法,对各种人群样品的淋巴细胞亚群进行计数。结果:细胞芯片法和流式细胞法所测定的CD3+、CD4+和CD8+数值,两种方法之间的相关性良好,相关系数r分别为0.98,0.93和0.95,平均误差:CD3+T细胞为-16个细胞/μl(P<0.001),CD4+T细胞为-8个细胞/μl(P<0.001),CD8+T细胞-10个细胞/μl(P<0.001)。结论:细胞芯片法可准确计数人类血液中的CD3+、CD4+和CD8+淋巴细胞,设备简单,价格低廉,操作简单快速,适用于基层医院。Objective： To compare the count accuracies of lymphocyte subpopulations （ CD3^＋＇ CD4^＋ and CD8^＋ ） detection with cell chip and flow cytometry. Methods： The methods of cell chip and flow cytometry were respectively used to count the lymphocyte subpopulations of various population samples. Results： There was a good correlation between the CD3^＋ , CD4^＋ and CD8 ^＋ values determined with cell chip and flow cytometry, the correlation coefficient r =0.98,0.93 and 0.95, with an average error： CD3^＋ T cells to - 16 cells/μl （ P 〈 0. 001 ）, CD4^＋ T cells -8 cells/μl （P 〈0.001）, and CD8^＋ T cells - 10 cells/μl （P 〈0.001）. Conclusion： Cell chip can accurately count the human blood CD3^＋ CD4^＋ and CD8 ^＋ lymphocytes, the device is simple, inexpensive, easy to operate and fast, suitable for primary hospital.

关 键 词：细胞芯片技术 淋巴细胞亚群 流式细胞法 

分 类 号：R446.6[医药卫生—诊断学]