肺表面活性物质蛋白B mRNA表达与新生儿呼吸窘迫综合征关联性研究  

Correlation between surfactant protein B mRNA expression and neonatal respiratory distress syndrome

作　　者：范含笑[1,2] 韩笑[1] 谢露[2] 曲雯雯[1] 尹晓娟[1,2] 

机构地区：[1]北京军区总医院附属八一儿童医院 [2]安徽医科大学北京军区总医院临床学院新生儿科,北京100700

出　　处：《中国优生与遗传杂志》2013年第4期39-41,F0002,共4页Chinese Journal of Birth Health & Heredity

基　　金：国家自然科学基金资助项目(30871397)

摘　　要：目的探讨肺表面活性物质蛋白B(SP-B)mRNA缺陷是否参与新生儿呼吸窘迫综合征(RDS)发病。方法选择无血缘关系的60例NRDS死亡患儿作为RDS组,其中≤32周组、32～36+6周组及≥37周组每组各纳入10例;选择无血缘关系的60例其他疾病新生儿死亡患儿作为对照组,胎龄与RDS组以1:1相匹配分成3亚组(P>0.05)。所有研究对象在死后半小时内取肺组织,然后将肺组织浸入4%多聚甲醛固定,石蜡包埋后5μm连续切片,采用原位杂交技术检测各组患儿SP-B mRNA在肺部的表达;计算两组SP-B mRNA缺陷的频率。结果 SP-B mRNA位于细胞胞浆中,随组别、胎龄、疾病严重性而变化;RDS组中,肺部SP-B mRNA表达阳性细胞数未随胎龄增加而增加,而对照组肺部SP-BmRNA表达阳性细胞数随胎龄增加而增加(F=50.124,P<0.001)。RDS组SP-B mRNA阳性表达细胞明显低于对照组(t=7.812,P<0.001)。NRDS组SP-B缺陷35例,缺陷频率58.3%,对照组SP-B缺陷8例,缺陷频率13.3%,RDS组高于对照组(χ2=26.421,P<0.001)。结论 SP-B mRNA缺陷参与了RDS的发病。Objective ： To investigate whether surfactant - associated protein B （ SP - B） mRNA deficiency is involved in the pathogenesis of neonatal respiratory distress syndrome （RDS）. Methods： A total of 60 unrelated neonates who died of RDS were recruited as the RDS group and then subgrouped into ≤32 weeks group, 32- 36＋6 weeks group and ≥37 weeks group （n = 10 per group） on the basis of gestational age. In addition, 60 neonates died of other diseases were enrolled as controls and subdivided into 3 matched groups based on the gestational age （P 〉 0. 05）. The lung tissues were collected within 30 min after death, fixed in 4% paraformalde- hyde, embedded in paraffin and consecutively cut into 5 - μm sections. In situ hybridization was done to detect the SP - B mRNA ex- pression in the lung and the frequency of SP - B mRNA deficiency was calculated. Results ： SP - B mRNA was mainly found in the cytoplasm and the SP - B mRNA level varied among neonates in different groups, with different gestational age and with different severi- ties of RDS. Among the RDS groups, the SP - B mRNA level was significantly higher than that in the control group （ t = 7. 812, P 〈 0. 001 ）, but that was comparable among RDS patients with different gestational ages （ F = 2. 348, P 〉 0. 105 ）. Among the control groups, the SP- B mRNA level increased with the increase in gestational age （F = 50. 124, P 〈 0. 001 ）. In the ≤32w group, the number of cells positive for SP- B mRNA in RDS patients was markedly reduced as compared to controls （t =3. 185, P 〈0. 01 ）. In the 32 - 36＋6w group, the number of cells positive for SP - B mRNA in RDS patients was significantly smaller than that in controls （ t =9. 342, P 〈0. 001 ）. In the ≥37w group, number of cells positive for SP - B mRNA in RDS patients was dramatically smaller than that in controls （ t = 4. 238, P 〈 0. 001 ）. Among RDS neonates, SP - B mRNA deficiency was noted in 35 patients with the frequency of 58. 3%. In the control gr

关 键 词：新生儿 呼吸窘迫综合征 肺表面活性物质蛋白B 信使RNA 缺陷 

分 类 号：R722.1[医药卫生—儿科]