Comparative analysis of intermuscular bones in fish of different ploidies  

作　　者：LI Ling ZHONG ZeZhou ZENG Ming LIU ShaoJun ZHOU Yi XIAO Jun WANG Jun LIU Yun 

机构地区：[1]Key Laboratory of Protein Chemistry and Developmental Biology of Ministry of Education of China, College of Life Sciences, Hunan Normal University

出　　处：《Science China(Life Sciences)》2013年第4期341-350,共10页中国科学（生命科学英文版）

基　　金：supported by Special Fund for Agro-scientific Research in the Public Interest (200903046);the National Natural Science Foundation of China (30930071);Major International Cooperation Projects of the National Natural Science Foundation of China (31210103918);Innovative Research Groups of Hunan Provincial Natural Science Fund (10JJ7004);the National High Technology Research and Development Program of China (2011AA100403);Pre-project of the National Key Basic Research and Development Program (2012CB722305);the Construct Program of the Key Discipline in Hunan Province;Construct Program of the National Key Discipline

摘　　要：We documented the number, morphology, and distribution of intermuscular bones in five fishes of different ploidy： Carassius auratus （Abbr.WCC, 2n=100）, Carassius auratus variety PengZe （Abbr.PZCC, 3n=150）, improved triploid crucian carp （Abbr.ITCC, 3n=150）, improved red crucian carp （Carassius auratus red var., Abbr.IRCC, , 2n=100）, and improved allotet- raploids （Abbr.GAT, （, 4n=200）. The number of intermuscular bones in WCC, PZCC, and GxAT ranged from 78 to 83 （n =81）, 80 to 86 （n =84）, and 77 to 84 （n=82）, respectively. The numbers in ITCC and IRCC were significantly lower, ranging from 77 to 82 （ n =79） and 58 to 77 （ n =71）, respectively. The average number of intermuscular bones in each sarco- mere, ranked in order from highest to lowest, was 0.721 （WCC）, 0.673 （PZCC）, 0.653 （GAT）, 0.633 （ITCC）, and 0.608 （IRCC）. There was no difference between ITCC and GxAT or between GxAT and PZCC. However, the average number of intermuscular bones in the sarcomeres of ITCC, WCC, and PZCC differed significantly, as did that of IRCC and the four other kinds of fish. The intermuscular bone of these five fishes was divided into seven shape categories, non-forked （I）, one-end-unequal-bi-fork （）, one-end-equal-bi-fork （Y）, one-end-multi-fork, two-end-bi-fork, two-end-multi-fork, and tree- branch types. Generally, the morphological complexity was higher in the anterior intermuscular bones than in the posterior body. The number of intermuscular bones was similar but not equal between the left and right sides of the body. ITCC had sig- nificantly fewer intermuscular bones than either WCC or PZCC, making it of greater commercial value. Additionally, IRCC and ITCC had fewer intermuscular bones than WCC. Our observations are significant in both fish bone developmental biology and genetic breeding.We documented the number, morphology, and distribution of intermuscular bones in five fishes of different ploidy: Carassius auratus (Abbr.WCC, 2n=100), Carassius auratus variety PengZe (Abbr.PZCC, 3n=150), improved triploid crucian carp (Abbr.ITCC, 3n=150), improved red crucian carp (Carassius auratus red var., Abbr.IRCC, ♀, 2n=100), and improved allotetraploids (Abbr.G×AT, ♂, 4n=200). The number of intermuscular bones in WCC, PZCC, and G×AT ranged from 78 to 83 ( =81), 80 to 86 ( =84), and 77 to 84 (=82), respectively. The numbers in ITCC and IRCC were significantly lower, ranging from 77 to 82 (=79) and 58 to 77 (=71), respectively. The average number of intermuscular bones in each sarcomere, ranked in order from highest to lowest, was 0.721 (WCC), 0.673 (PZCC), 0.653 (G×AT), 0.633 (ITCC), and 0.608 (IRCC). There was no difference between ITCC and G×AT or between G×AT and PZCC. However, the average number of intermuscular bones in the sarcomeres of ITCC, WCC, and PZCC differed significantly, as did that of IRCC and the four other kinds of fish. The intermuscular bone of these five fishes was divided into seven shape categories, non-forked (I), one-end-unequal-bi-fork (卜), one-end-equal-bi-fork (Y), one-end-multi-fork, two-end-bi-fork, two-end-multi-fork, and treebranch types. Generally, the morphological complexity was higher in the anterior intermuscular bones than in the posterior body. The number of intermuscular bones was similar but not equal between the left and right sides of the body. ITCC had significantly fewer intermuscular bones than either WCC or PZCC, making it of greater commercial value. Additionally, IRCC and ITCC had fewer intermuscular bones than WCC. Our observations are significant in both fish bone developmental biology and genetic breeding.

关 键 词：intermuscular bone epineural bone epipleural bone MORPHOLOGY genetic breeding 

分 类 号：S917.4[农业科学—水产科学]