BmCyclin B and BmCyclin B3 are required for cell cycle progression in the silkworm, Bombyx mori  

作　　者：PAN MinHui HONG KaiLi CHEN XiangYun PAN Chun CHEN XueMei KUANG XiuXiu LU Cheng 

机构地区：[1]State Key Laboratory of Silkworm Genome Biology, Southwest University

出　　处：《Science China(Life Sciences)》2013年第4期360-365,共6页中国科学（生命科学英文版）

基　　金：supported by the National Natural Science Foundation of China (31272505, 31172269)

摘　　要：Cyclin B is an important regulator of the cell cycle G2 to M phase transition. The silkworm genomic database shows that there are two Cyclin B genes in the silkworm (Bombyx mori), BmCyclin B and BmCyclin B3. Using silkworm EST data, the cyclin B3 (EU074796) gene was cloned. Its complete cDNA was 1665 bp with an ORF of 1536 bp derived from seven exons and six introns. The BmCyclin B3 gene encodes 511 amino acids, and the predicted molecular weight is 57.8 kD with an isoelectric point of 9.18. The protein contains one protein damage box and two cyclin boxes. RNA interference-mediated reduction of BmCyclin B and BmCyclin B3 expression induced cell cycle arrest in G2 or M phase in BmN-SWU1 cells, thus inhibiting cell proliferation. These results suggest that BmCyclin B and BmCyclin B3 are necessary for completing the cell cycle in silkworm cells.Cyclin B is an important regulator of the cell cycle G2 to M phase transition. The silkworm genomic database shows that there are two Cyclin B genes in the silkworm （Bombyx mori）, BmCyclin B and BmCyclin B3. Using silkworm EST data, the cyclin B3 （EU074796） gene was cloned. Its complete cDNA was 1665 bp with an ORF of 1536 bp derived from seven exons and six introns. The BmCyclin B3 gene encodes 511 amino acids, and the predicted molecular weight is 57.8 kD with an isoelectric point of 9.18. The protein contains one protein damage box and two cyclin boxes. RNA interference-mediated reduction of BmCyclin B and BmCyclin B3 expression induced cell cycle arrest in G2 or M phase in BmN-SWU1 cells, thus inhibiting cell proliferation. These results suggest that BmCyclin B and BmCyclin B3 are necessary for completing the cell cycle in silkworm cells.

关 键 词：Bombyx mori cell cycle BmCyclin B BmCyclin B3 cell proliferation 

分 类 号：Q25[生物学—细胞生物学]