A microRNA encoded by HSV-1 inhibits a cellular transcriptional repressor of viral immediate early and early genes  

作　　者：WU WenJuan GUO ZhongPing ZHANG XueMei GUO Lei LIU LongDing LIAO Yun WANG JingJing WANG LiChun LI QiHan 

机构地区：[1]Institute of Medical Biology, Chinese Academy of Medical Sciences, Peking Union Medical College [2]First Affilliated Hospital of Kunming Medical University [3]Chinese Pharmacopoeia Commission

出　　处：《Science China(Life Sciences)》2013年第4期373-383,共11页中国科学（生命科学英文版）

基　　金：supported by the National Natural Science Foundation of China (30670094, 30700028);National Basic Research Program of China (2012CB518901, 2011CB504903)

摘　　要：Viral microRNAs are one component of the RNA interference phenomenon generated during viral infection. They were first identified in the Herpesviridae family, where they were found to regulate viral mRNA translation. In addition, prior work has suggested that Kaposi＇s sarcoma-associated herpesvirus （KSHV） is capable of regulating cellular gene transcription by miRNA. We demonstrate that a miRNA, hsvl-mir-H27, encoded within the genome of herpes simplex virus 1 （HSV-1）, targets the mRNA of the cellular transcriptional repressor Kelch-like 24 （KLHL24） that inhibits transcriptional efficiency of viral imme- diate-early and early genes. The viral miRNA is able to block the expression of KLHL24 in cells infected by HSV-1. Our dis- covery reveals an effective viral strategy for evading host cell defenses and supporting the efficient replication and prolifera- tion of HSV- 1.Viral microRNAs are one component of the RNA interference phenomenon generated during viral infection. They were first identified in the Herpesviridae family, where they were found to regulate viral mRNA translation. In addition, prior work has suggested that Kaposi's sarcoma-associated herpesvirus (KSHV) is capable of regulating cellular gene transcription by miRNA. We demonstrate that a miRNA, hsv1-mir-H27, encoded within the genome of herpes simplex virus 1 (HSV-1), targets the mRNA of the cellular transcriptional repressor Kelch-like 24 (KLHL24) that inhibits transcriptional efficiency of viral immediate-early and early genes. The viral miRNA is able to block the expression of KLHL24 in cells infected by HSV-1. Our discovery reveals an effective viral strategy for evading host cell defenses and supporting the efficient replication and proliferation of HSV-1.

关 键 词：herpes simplex virus I （HSV-1） microRNAs （miRNAs） cellular transcriptional repressor Kelch-like 24 

分 类 号：R373[医药卫生—病原生物学]