多菌灵降解菌GRPD-1的分离鉴定及降解特性研究  

Isolation,Identification and Characterization of a Carbendazim-degrading Bacterial GRPD-1

作　　者：蒲丹[1] 张晓喻[1,2] 刘刚[1,2] 黄春萍[1,2] 李琪[1,2] 张宏[1,2] 

机构地区：[1]四川师范大学生命科学学院,四川成都610101 [2]四川师范大学植物资源应用与开发研究所,四川成都610101

出　　处：《四川师范大学学报（自然科学版）》2013年第2期289-295,共7页Journal of Sichuan Normal University（Natural Science）

基　　金：四川省科技厅青年基金(2011JY0104)资助项目;四川师范大学开放性仪器基金

摘　　要：从成都彭州蔬菜基地土壤中分离得到1株能以多菌灵作为唯一碳氮源生长的细菌,命名为GRPD-1.经形态观察、生理生化实验以及16S rDNA基因同源性序列分析,鉴定其为假单胞菌属(Pseudo-monas sp.).研究了该菌株在不同pH值、温度、接种量和外加碳氮源条件下对多菌灵降解效果的影响.实验结果表明,该菌株在以多菌灵为唯一碳氮源的基础盐培养基中培养6 d,对50 mg/L多菌灵的降解率达到60%.添加少量葡萄糖、蛋白胨作额外碳氮源时可促进菌株GRPD-1对多菌灵的降解,第2天的降解率提高到90%以上.其最适降解条件为pH值7.0,温度30℃,接种量9%.研究结果表明菌株GRPD-1在农药污染的土壤修复方面具有广阔的应用前景.还考察了菌株在不同碳氮源生长条件下产生的蛋白酶的试验,通过聚丙烯酰胺凝胶电泳,初步分析了酶谱条带,发现菌株在不同碳氮源生长条件下表达的蛋白酶有差异.A bacterial strain GRPD-1, capable of utilizing carbendazim as sole carbon and nitrogen source, was isolated from Pengzhou vegetable base of Chengdu city. According to its morphological, physiological and biochemical analysis and the analysis of its 16S rDNA gene sequence, this strain was identified as Pseudomonas sp. , named as GRPD-1. The biodegradation efiqciency of carbendazim by strain GRPD-1 in different conditions was investigated. Results indicated under the condition of incubation in mineral salt medium GRPD-1 could degrade 60% of 50 mg/L carbendazim in 6 days. Culture medium with glucose, peptone could increase carbendazim degradation rate to more than 90% in 2 days. The optimal pH, temperature and inoculation amount for the degradation were 7.0, 30℃ and 9%, respectively. The results showed that the strain GRPD-1 had a good application prospect in control of carbendazim pollution in the soil. The effect of different carbon sources on the strain was studied. And the protein channel of GRPD-1 by SDS-polyacryl- amide gel electrophoresis was analysed. The result indicated the different carbon and nitrogen source leaded to express different proteins.

关 键 词：多菌灵 假单胞菌属 降解特性 聚丙烯酰胺凝胶电泳 

分 类 号：Q936[生物学—微生物学]