麦洼牦牛乳酸脱氢酶-B基因多态性的PCR-RFLP分析  

PCR-RFLP Analysis of Polymorphisms of Lactate Dehydrogenase B in Maiwa Yak

作　　者：马晓琴[1] 黄林[1] 刘薇[1] 刘浩浩[1] 陈洁玲[1] 郑玉才[1] 

机构地区：[1]西南民族大学生命科学与技术学院,成都610041

出　　处：《四川动物》2013年第2期192-194,共3页Sichuan Journal of Zoology

基　　金：国家973计划前期研究专项(2007CB116204)

摘　　要：为了从分子水平上检测麦洼牦牛Bos grunniens乳酸脱氢酶-1(LDH1)的H亚基编码基因Ldhb的多态性,实验提取79头麦洼牦牛基因组DNA,采用PCR-RFLP技术分析Ldhb基因多态性,并测定牦牛背最长肌中肌红蛋白含量。实验建立的牦牛Ldhb的G896A突变位点的PCR-RFLP检测方法,在79头麦洼牦牛中检测到Ldhb-AA和Ldhb-AG两种基因型,其中AG基因型频率为16.46%,并且这些样品的乳酸脱氢酶-1的电泳迁移率快于Ldhb-AA样品。本实验未检测到Ldhb-GG基因型;麦洼牦牛Ldhb基因多态性与背最长肌中的肌红蛋白含量之间未见相关性。In order to study the polymorphism of Ldhb gene which encodes H-subunit of lactate dehydrogenase-1（LDH1） in yak（Bos grunniens） at molecular level,the genomic DNA was extracted from 79 Maiwa yaks with 5～7 tandem repeats,and PCR-RFLP method was applied to analyze the genetic polymorphism of Ldhb,meanwhile,myoglobin content was determined in longissimus dorsi muscle.PCR-RFLP method was used to discover the mutation of G896A of yak Ldhb,and two genotypes（Ldhb-AA and Ldhb-AG） were found in 79 Maiwa yaks,genotype frequency of Ldhb-AG was 16.46%.Furthermore,these samples showed faster migration rate of lactate dehydrogenase-1 than the samples carrying Ldhb-AA during electrophoresis.Ldhb-GG genotype was not observed in this experiment.No significant correlation showed between Ldhb polymorphism and myoglobin content in longissimus dorsi muscles of Maiwa yaks.

关 键 词：牦牛 乳酸脱氢酶 多态性 PCR-RFLP 肌红蛋白 

分 类 号：S823[农业科学—畜牧学]