川产叠鞘石斛与石斛药典收载品种指纹图谱对比研究  

A Compairison Investigation between Dendrobium Aurantiacum Rchb.f.var. Denneanum(Kerr.)Z.H.Tsi and Pharmacopeia Species

作　　者：许莉[1] 郭力[1] 陈佳江[1] 韦练[1] 罗方利[1] 张廷模[1] 

机构地区：[1]成都中医药大学,四川成都611137

出　　处：《成都中医药大学学报》2013年第1期8-10,52,共4页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：科技部"十二五"科技支撑项目(编号:20011BAI13B02-8)

摘　　要：目的:对叠鞘石斛与石斛药典收载品种金钗、鼓槌和流苏石斛HPLC-DAD-ELSD指纹图谱进行对比研究。方法:采用Dikma C18(250 mm×4.6 mm,5μm)色谱柱;乙腈-0.5%冰醋酸水溶液梯度洗脱;柱温:30℃;流速:1 mL/min;DAD串联蒸发光散射检测器检测。结果:首次建立了石斛的HPLC-DAD-ELSD指纹图谱鉴别方法,并对9个指纹峰进行了标定。首次对叠鞘石斛和石斛药典收载品种金钗、鼓槌、流苏石斛指纹图谱进行对比研究。结论:建立的方法稳定、可靠、重现性好,可直接了解石斛品种与化学成分变化的关系。叠鞘石斛与石斛药典收载品种有一定相似性,但相似度较低。Objective：To set up the fingerprint chromatograms of chemical principles of Dendrobii Caulis by HPLC-DAD-ELSD.Methods：Analysis was performed at 30℃ on a Dikma Cl8 column（4.6mm×250mm,5μm）with a mobile phase consisting of acetonitrile-0.5% glacial acetic acid,gradient eluent,at the flow rate of 1.0 mL/min with DAD and ELSD detection.Results：HPLC-DAD-ELSD fingerprint of Dendrobii Caulis was established,and we have pinpointer 9 peaks based on it.Conclusion：The method was stable,reliable and repeatable.It＇s useful for the study on the relationship between chemical components and species of Dendrobii Cauli.Similarity among different species was low.

关 键 词：叠鞘石斛 指纹图谱 HPLC-DAD-ELSD联用 

分 类 号：R282.71[医药卫生—中药学]