银翘解毒丸对流感病毒感染小鼠肺组织β-defensin1表达的影响  

Effect of Yin-Qiao-Jie-Du Pill on β-defensin1's Expression in Lung Tissue of Mice Infected With Influenza A Virus

作　　者：杨红亚[1] 张天娥[1] 刘伟伟[1] 徐中环[1] 丁维俊[1] 

机构地区：[1]成都中医药大学基础医学院,四川成都610075

出　　处：《成都中医药大学学报》2013年第1期33-36,共4页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：四川省教育厅自然基金项目(编号:12ZB213);成都中医药大学科技发展基金项目(编号:ZRYB2007020)

摘　　要：目的:研究银翘解毒丸对流感病毒感染模型小鼠肺组织β-防御素1(β-defensin1)表达的影响,从诱生抗菌肽层面探讨其抗病毒活性。方法:以甲型流感病毒鼠肺适应株(FM1)感染小鼠为模型,采用银翘解毒丸灌胃治疗,用实时荧光定量PCR技术(Real-time quantitative PCR,qPCR)分别检测感染后第1、3、7 d小鼠肺组织β-defensin1的表达。结果:银翘解毒丸治疗组小鼠β-defensin1的表达在感染后的第1 d、第3 d均高于模型组,第7 d的表达低于模型组,其中第3 d表达的差异有显著性意义(P<0.01)。在感染后第1～8 d内β-defensin1表达呈现先升高后降低的变化,在感染后的第4 d呈现高峰。结论:银翘解毒丸可在流感病毒感染早期增加小鼠肺组织β-defensin1 mRNA的表达。Objective： To study the effect of Yin-qiao-jie-du pill on β-defensin1＇s expression in the lung tissue of mice infected with influenza virus,and to investigate its antiviral effect from antibacterial peptide perspective.Methods：Mice infected with influenza virus A adapted strains of rat lung（FM1） were selected as animal model.The infected mice were dosed daily by gastric gavage with Yin-qiao-jie-du pill.Expression of β-defensin1 in the lung tissue of mice was detected with real-time quantitative PCR after being infected for 1day,3 days,and 7 days.Results： The level of β-defensin1 mRNA expression in the lung tissue of mice after being infected for 1day and 3 days was higher,compared with that of model group.The gene expression level was lower after being infected for 7 days.There was a significant difference between the expression level of the lung tissue of mice in being infected for 3 days group and model group（P0.01）.From 1st to 8th day after infection,the gene expression showed increasing firstly and then decreasing trend,and on the 4th day after infection in the peak.Conclusion：Yin-qiao-jie-du pill can increase β-defensin1 mRNA expression in lung tissue of mice that infected with influenza virus A in early stage.

关 键 词：银翘解毒丸 流感病毒 β-防御素1 实时荧光定量PCR 

分 类 号：R289[医药卫生—方剂学] R373.1[医药卫生—中药学]