Chromatographic fingerprint analysis of Bupleuri Radix by HPLC-ELSD  

柴胡的HPLC-ELSD指纹图谱研究(英文)

作　　者：周勇[1,2] 王延亮 马志桥 王邠 梁鸿[1,2] 赵玉英 张庆英[1,2] 

机构地区：[1]北京大学医学部天然药物及仿生药物国家重点实验室 [2]药学院天然药物学系,北京100191

出　　处：《Journal of Chinese Pharmaceutical Sciences》2013年第1期40-46,共7页中国药学（英文版）

基　　金：The project of Quality Standards for Chinese Medicines and Information System Platform (Grant No.2009ZX09308004);National Natural Science Foundation of China (Grant No.21172008)

摘　　要：To facilitate the species identification and quality assessment of Chaihu （Bupleuri Radix）, a simple and valid chromatographic fingerprint method was developed. The method uses high-performance liquid chromatography coupled with evaporative light scattering detector （HPLC-ELSD） and the data analysis is assisted by professional analytical software recommended by the State Food and Drug Administration （SFDA）. The results indicate that Nan Chaihu raw materials and Chaihu decoction pieces vary markedly in chemical quality, while Bei Chaihu raw materials are relatively more stable. Furthermore, it is obvious that Nan Chaihu is chemically very different from Bei Chaihu, suggesting that Nan Chaihu may not be suitable for medicinal use. In addition, the obvious differences between the chromatograms of decoction pieces and raw materials, especially the peaks between 30 and 40 rain and after 45 rain, indicate possible effects of the processing procedures on the chemicals. By analyzing the fingerprints of all samples, 12 main saponin-like fingerprint peaks, of which at least three are characteristic peaks of saikosaponins a, c, and d, are proposed to be considered for further characterization and quality evaluation of Chaihu.本文建立了一种简单、有效的柴胡药材的高效液相色谱-蒸发光散射(HPLC-ELSD)指纹图谱方法,并对不同产地和不同品种的柴胡药材及其饮片进行了系统评价。研究结果显示南柴胡药材和市售柴胡饮片的化学成分变化较大,而北柴胡药材的化学成分则相对稳定,且南柴胡药材与北柴胡药材化学成分差别明显,提示南柴胡药材作为柴胡应用要谨慎。另外与柴胡药材相比,柴胡饮片的指纹图谱也发生了变化,尤其是保留时间在30–40分钟之间和45分钟之后色谱峰变化尤为明显,提示在炮制过程中可能发生了化学成分的变化。最终本文确定了柴胡中12个主要皂苷类成分的色谱指纹峰,并建议柴胡皂苷a、c和d为其基本特征峰,这对于柴胡的品种鉴定和质量控制具有指导意义。

关 键 词：Bupleuri Radix Bupleurum chinense Bupleurum scorzonerifolium Chromatographic fingerprint HPLC-ELSD SAIKOSAPONINS 

分 类 号：R284.1[医药卫生—中药学]