Fingerprint analysis of Ophiopogonis Radix by HPLC-UV-ELSD coupled with chemometrics methods  

HPLC-UV-ELSD结合化学计量学方法研究麦冬的指纹图谱(英文)

作　　者：李宁[1] 车彦云[1] 张梁[1] 张加余[1] 周渊[1] 姜勇[1] 屠鹏飞[1] 

机构地区：[1]北京大学医学部药学院天然药物及仿生药物国家重点实验室,北京100191

出　　处：《Journal of Chinese Pharmaceutical Sciences》2013年第1期55-63,共9页中国药学（英文版）

基　　金：National Key Technology R&D Program"New Drug Innovation" of China (Grant No. 2012ZX09304-005,2012ZX09301002-002-002)

摘　　要：A new, simple and reliable method using HPLC-UV-ELSD was developed to generate the fingerprint of Ophiopogonis Radix. Homoisoflavonoids and steroidal saponins were determined simultaneously in a single run. A total of 27 Ophiopogonis Radix samples were analyzed, and 18 reference substances were used for the identification of the common peaks. The fingerprint was further analyzed by chemometrics methods including similarity analysis （SA）, hierarchical clustering analysis （HCA） and principal component analysis （PCA）. The results indicated that the combination of chromatographic fingerprint and chemometrics analysis could be used for the geographical differentiation and quality evaluation of Ophiopognnis Radix.本文利用HPLC-UV-ELSD技术建立了一种新的麦冬化学指纹图谱分析方法。该方法简单、可靠,可同时对高异黄酮及甾体皂苷两类成分进行检测。在此基础上,对27批麦冬药材的指纹图谱进行了分析。采用18个对照品对部分共有峰进行了指认。采用相似度分析、聚类分析、主成分分析等化学计量学方法对指纹图谱进行了进一步分析。结果显示,色谱指纹图谱结合化学计量学方法可用于不同产地麦冬的鉴定及药材的质量评价。

关 键 词：Ophiopogonis Radix Ophiopogonjaponicus HPLC-UV-ELSD FINGERPRINT CHEMOMETRICS Quality evaluation 

分 类 号：R284[医药卫生—中药学]