UV-Vis测定芎姜复方CO_2超临界提取物中总内酯和总萜类的含量  

Determination of total lactones and total triterpenoids in CO_2-SFE of Xiongjiang mixture by UV-Vis spectrophotometry

作　　者：钦富华[1] 夏晓静[1] 董宇[2] 章建民[2] 张扬[2] 王明军[1] 

机构地区：[1]浙江医药高等专科学校,浙江宁波315100 [2]浙江省中医药研究院,杭州310007

出　　处：《中南药学》2013年第3期222-224,共3页Central South Pharmacy

基　　金：宁波市自然科学基金项目(No.2007A610078);浙江省自然科学基金项目(No.Y2080501)

摘　　要：目的建立测定芎姜复方CO2超临界提取物中总内酯和总萜类含量的方法。方法以洋川芎内酯A为对照,30 mg.mL-1的氢氧化钠溶液,30℃恒温反应30 min,于305 nm波长处测定吸光度,测定总内酯含量;以6-姜酚为对照,5%香草醛-冰乙酸溶液、高氯酸显色,于560 nm波长处测定吸收度,测定总萜类含量。结果洋川芎内酯A在0.062 5～1.0 mg.mL-1与吸收度呈良好线性关系(r=0.999 9),平均回收率为97.9%,RSD为2.7%;6-姜酚在0.03～0.4 mg.mL-1与吸收度呈良好线性关系(r=0.999 9),平均回收率为97.6%,RSD为3.6%。结论本方法简便,准确,可用于芎姜CO2超临界提取物及其制剂中总内酯和总萜类含量的快速测定。Objective To establish a UV-Vis spectrophotometry method for the determination of total lactones and triterpenoids in CO2-SFE of Xiongjiang mixture.Methods senkyunolide A as the control,the total lactones were measured by UV-Vis spectrophotometry with 30 mg.mL-1 sodium hydroxide solution and in 30 ℃ insulation reacting for 30 min,the absorbance was measured at 305 nm wavelength.with 6-gingerol as the control,the total triterpenoids were measured by UV-Vis spectrophotometry with 5% vanillin-glacial acetic acid solution and perchloric acid as the color-developing agent,detected at 560 nm wavelength.Results Senkyunolide A had good linearity（r = 0.999 9） at 0.062 5-1.0 mg.mL-1,the average recovery was 97.9%,with RSD of 2.7%,while 6-gingerol had good linearity（r = 0.999 9） at 0.035-0.40 mg.mL-1,the average recovery was 97.6%,with RSD of 3.6%.Conclusion The method is simple,accurate and can be used for the determination of total lactones and triterpenoids in CO2-SFE of Xiongjiang mixture.

关 键 词：紫外-可见分光光度法 芎姜复方 CO2超临界提取物 总内酯 总萜类 

分 类 号：R917[医药卫生—药物分析学] R927.2[医药卫生—药学]