Effects of electro-acupuncture on embryo implanted potential of patients with infertility of different syndromes  

电针对不同证型不孕症患者胚胎种植潜能的影响(英文)

作　　者：孔风云[1,2] 张琪瑶[1] 管群[1] 菅福琴[1] 孙伟[1] 王越[2] JIAO Yue 

机构地区：[1]山东中医药大学第二附属医院生殖科 [2]山东大学医学院

出　　处：《World Journal of Acupuncture-Moxibustion》2012年第4期32-36,共5页世界针灸杂志（英文版）

基　　金：Supported by ordinary item of scienceand technology development plan of TCM in Shandong Province:2009-150

摘　　要：Objective To explore the impacts of electro-acupuncture on embryo implanted potential and its molecular mechanism in patients with infertility of different syndromes. Methods Among infertile patients treated with in vitro fertilization and embryo transplantation （IVF-ET） combined with electro-acupuncture, 82 cases of kidney deficiency syndrome （group A）, 74 cases of liver qi stagnation syndrome （group B） and 54 cases of phlegm dampness syndrome （group C） were selected. Patients in three groups all received a long program of ovarian hyperstimulation. Additionally, electro-acupuncture was applied before controlled ovarian hyperstimulation （COH） and in the process of ovarian hyperstimulation. Contents of human leukocyte antigen G （HLA-G） in the serum were determined in three groups respectively on the 2nd day of menstruation （M2） and the day of human Chorionic Gonadotropin （hCG） injection. Content of HLA-G in the culture solution of three groups was examined separately on the day of embryo transplantation. The fertilization rate, implantation rate and clinical pregnancy rate were observed for patients in three groups. Results Contents of HLA-G in the serum on the day of hCG injection and in the culture solution on the day of embryo transplantation in group A and B were significantly higher than those in group C （all P0.05）. However, there was no significant difference with contents of HLA-G in serum on M2 day among three groups. The high quality embryo rate in either group A （73.6%, 352/478） or group B （70.6%, 379/537） was significantly higher than that in group C （54.2%, 208/384） with significantly statistical difference （all P0.01）. But there were no significant differences in clinical pregnancy rate, fertilization rate and cleavage rate among three groups. Conclusion Electro-acupuncture combined could increase contents of HLA-G in the body and the level of HLA-G secreted from embryos of patients in the process of IVF-ET. Eventually, the pregnancy outcome and the目的:探讨电针对不同证型不孕症患者胚胎种植潜能的影响,并研究其作用的分子机制。方法:在配合电针进行体外受精胚胎移植(IVF-ET)的不孕症患者中选取肾虚型(A组)82例、肝郁型(B组)74例、痰湿型(C组)54例,3组患者均为长方案超促排卵,在控制性超排卵前及超排卵过程中配合电针,测定3组患者月经第2日(M2)、绒促腺素(hCG)注射日血清和胚胎移植日培养基中人类白细胞抗原G(HLA-G)的含量,观察3组患者的受精率、种植率和临床妊娠率。结果:A、B两组hCG日血清HLA-G含量和移植日培养基HLA-G含量均显著高于C组(均P<0.05),但3组M2日血清HLA-G含量差异无统计学意义。A、B两组优质胚胎率为73.6%(352/478)、70.6%(379/537),显著高于C组的54.2%(208/384),差异均有统计学意义(均P<0.01);3组的临床妊娠率、受精率和卵裂率差异均无统计学意义。结论:在IVF-ET过程中配合电针治疗可通过促进患者体内HLA-G含量升高,提高胚胎分泌的HLA-G的水平,进而改善患者的妊娠情况,提高妊娠率。肾虚型和肝郁型两种不孕症患者经电针治疗后其临床结局较痰湿型效果好。

关 键 词：INFERTILITY female ELECTRO-ACUPUNCTURE in vitro fertilization（IVF） embryo transplantation （ET） syndrome differentiatio 

分 类 号：R246.3[医药卫生—针灸推拿学]