重组人促红细胞生成素对脑创伤大鼠神经功能和空间记忆的影响  

Effects of rhEPO on neurol function and spatial memory in traumatic brain injury rats

作　　者：王亮[1] 王晓楠[2] 王东[3] 尉辉杰[3] 刘晓智[1] 刘振林[1] 张建宁[3] 

机构地区：[1]天津市第五中心医院,天津300450 [2]天津市南开医院 [3]天津医科大学总医院

出　　处：《山东医药》2012年第36期37-39,104,共4页Shandong Medical Journal

基　　金：天津市自然科学基金面上项目(09JCYBJC09500);天津市卫生局科技攻关项目(10KG210)

摘　　要：目的研究重组人促红细胞生成素(rhEPO)对颅脑创伤大鼠行为学和神经功能恢复的影响。方法 30只成年雄性Wistar大鼠,随机分为假手术组、生理盐水对照组和rhEPO治疗组。治疗组大鼠于打击后半小时内腹腔注射rhEPO 5 000 U,连续注射5 d,对照组打击后半小时内腹腔内注射等量生理盐水。于术后1、4、7、14、21、25 d对3组大鼠神经功能进行mNSS评分。术后21～25 d进行水迷宫实验,记录大鼠每日逃避潜伏期和目标象限百分率。结果 mNSS评分结果显示,与生理盐水治疗组比较,rhEPO治疗组大鼠于伤后第14、21、25天其行为学功能得到明显改善(P<0.05)。rhEPO治疗组大鼠伤后24和25 d的象限百分率明显比生理盐水治疗组大鼠升高(P<0.05),逃避潜伏期短于生理盐水治疗组(P<0.05)。结论 rhEPO对脑创伤大鼠行为学功能和学习及记忆功能的恢复有明显的促进作用。Objective To research the effect of rhEPO on improving neural function and spatial memory recovery in traumatic injury （TBI） rats. Methods Thirty male Wistar rats were randomly devided into sham-operated group, traumat- ic brain injury with saline-treated group （TBI ＋ saline group） and TBI with recombinant human erythropoietin group （TBI ＋ rhEPO group）. Each rat in the TBI ＋ rhEPO group received rhEPO intraperitonieally injection in half an hour after TBI at a dose of 5 000 U/（ kg·d） for 5 consecutive days. While the rats in the TBI ＋ saline group only received the saline injec- tion with the eaual volumne. The mNSS was used to evaluate the functional recovery induced by rhEPO at 1,4, 7, 14, 21, 25 day after TBI. The Morris water maze was used for investigating the memory recovery at 21-25 days post-TBI, and the escape latency and percentage of target quadrant were recorded. Results The mNSS showed rhEPO treated TBI rats signif- icantly improved at 14, 21, 25 day after TBI, and the spatial learning ability was significantly improved in rhEPO-treated rats at 24 and 25 day after TBI （ P 〈 0.05 ）, compared with the saline-treated rats. Condusion rhEPO could enhance the neuron function and spatial memory ability recovery in TBI rats.

关 键 词：重组人促红细胞生成素 脑创伤 水迷宫 

分 类 号：R332[医药卫生—人体生理学]