提取剂及提取部位对兔防御素提取效果的影响  

Effects of Extract Solvent and Position on Hare Alexin Extraction

作　　者：张剑云[1] 杨丽娟[2] 王琦[2] 

机构地区：[1]塔里木大学生命科学学院,新疆阿拉尔843300 [2]塔里木大学动物科技学院,新疆阿拉尔843300

出　　处：《湖北农业科学》2012年第9期1851-1852,1882,共3页Hubei Agricultural Sciences

摘　　要：腹腔注射大肠杆菌刺激成年健康塔里木兔(Lepus yarkandensis)和家兔(L.capensis),取腹水和圆小囊组织,经沸水浴处理后,分别以10倍体积的1 mL/L的乙酸或50 mmol/L Tris-HCl为提取剂提取防御素,提取液用Sephadex G-50凝胶柱过滤层析,产物经茚三酮染色鉴定,并用Folin-酚试剂法检测防御素含量。结果表明获得了纯化的兔防御素,其中以Tris-HCl为提取剂,从塔里木兔圆小囊中提取所得防御素含量最高。After injection of Escherichia coli in enterocoelia,ascites and sacculus rotundus of adult health Lepus yarkandensis and L.capensis was collected and treated by boiling water.Alexin was extract using 1 mL/L acetic acid or 50 mmol/L Tris-HCl as solvent.The extractant was purified by Sephadex G-50 gel column chromatography filter,and tested by ninhydrin and folin-phenol method.The results showed that purified alexin was obtained;and the content of alexin extracted by Tris-HCl from sacculus rotundus was the highest.

关 键 词：兔 防御素 提取 

分 类 号：TQ465.6[化学工程—制药化工]