表达日本血吸虫凋亡抑制因子的重组腺病毒的构建和鉴定  

CONSTRUCTION AND CHARACTERIZATION OF A RECOMBINANT ADENOVIRUS FOR EXPRESSING INHIBITOR APOPTOSIS PROTEIN OF SCHISTOSOMA JAPONICUM

作　　者：程国锋[1] 卫大群 罗荣[1] 石耀军[1] 

机构地区：[1]中国农业科学院上海兽医研究所农业部动物寄生虫学重点开放实验室,上海200241 [2]河北省滦县畜牧水产局,滦县063700

出　　处：《中国动物传染病学报》2012年第3期31-35,共5页Chinese Journal of Animal Infectious Diseases

基　　金：上海市科学技术委员会项目(10410703400和10PJ1412300)

摘　　要：为探讨腺病毒介导的基因操作技术在血吸虫基因功能中的应用,本研究构建了表达日本血吸虫凋亡抑制因子(aninhibitor apoptosis protein of Schistosoma japonicum,SjIAP)的腺病毒,免疫印迹分析表明获得了表达SjIAP的重组病毒,并对重组病毒的滴度进行了测定。本研究为进一步利用腺病毒对血吸虫基因操作相关研究及SjIAP的功能奠定了基础。To investigate adenovirus mediated gene modulation for Schistosomajaponicum and further determine the function of inhibitor apoptosis protein of Schistosoma japonicum （SjlAP）, we constructed an adenoviral vector expressing SjlAP. Western blot analysis indicated that SjlAP was expressed in the recombinant adenovirns. The recombinant adenovirus was grown in HEK293 cells and the titer was determined using Spearman-Karber method. Overall, data gathered from the present study can be used in future studies on Adenovirns mediated gene modulation and flmctions of SjIAP in Schistosomajaponicum.

关 键 词：日本血吸虫 基因操作 腺病毒 凋亡抑制因子 

分 类 号：S852.735[农业科学—基础兽医学] Q591.2[农业科学—兽医学]