日本血吸虫WD40信号蛋白的原核表达  

CLONING AND PRELIMINARY ANALYSIS OF A WD40 SIGNAL MOLECULE FROM SCHISTOSOMA JAPONICUM

作　　者：罗荣[1] 周春景[1] 石耀军[1] 赵江平[1] 程国锋[1] 

机构地区：[1]中国农业科学院上海兽医研究所农业部动物寄生虫学重点开放实验室,上海200241

出　　处：《中国动物传染病学报》2012年第3期50-54,共5页Chinese Journal of Animal Infectious Diseases

基　　金：国家自然科学基金(30901068);上海市科学技术委员会项目(10410703400和10PJ1412300)

摘　　要：本研究克隆了编码日本血吸虫WD40信号蛋白的cDNA,并分析了WD40在日本血吸虫不同发育时期的转录情况。同时,还利用原核表达系统对该蛋白的部分序列进行了表达,并进行了重组蛋白的纯化和多克隆抗体的制备,Western blot分析表明本研究中获得多克隆抗体可与重组表达的蛋白和血吸虫全虫蛋白裂解物具有特异性反应。本试验结果为进一步研究WD40信号分子的功能奠定了初步基础。In the present study, the cDNA fragment encoding WD40 signal molecule in Schistosoma japonicum was amplified by using PCR technique and then subcloned into a pET28a（＋） vector for expressing recombinant protein （rSj3ND40）. The recombinant protein was subsequently purified by using a Nickel charged affinity column. The polyclonal antibodies against the WD40 protein were raised by immunizing the purified protein in Newland rabbit. Western blot analysis indicated that the polyclonal antibodies were able to specifically recognize the recombinant SjWD40 protein. Overall, the present study provides fundamental information for further investigating the functions of WD40 protein in Schistosomajaponicum.

关 键 词：日本血吸虫 信号分子 WD40 克隆 原核表达 

分 类 号：S852.735[农业科学—基础兽医学] Q591.2[农业科学—兽医学]