TGF-β1-induced Synthesis of Collagen Fibers in Skeletal Muscle-Derived Stem Cells  

作　　者：陈燕花 彭云龙 王旸 翁雨雄 李涛 张燕 陈振兵 

机构地区：[1]Department of Hand Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2013年第2期238-243,共6页华中科技大学学报（医学英德文版）

基　　金：supported by the National Natural Science Foundation of China(No.30872627)

摘　　要：The aim of this study was to investigate the mechanism of deposition of extracellular matrix induced by TGF-β1 in skeletal muscle-derived stem cells （MDSCs）. Rat skeletal MDSCs were obtained by using preplate technique, and divided into four groups： group A （control group）, group B （treated with TGF-β1, 10 ng/rnL）, group C （treated with TGF-β1 and anti-connective tissue growth factor （CTGF）, both in 10 ng/mL）, and group D （treated with anti-CTGF, 10 ng/mL）. The expression of CTGF, collagen type- I （COL- I ） and collagen type-III （COL-III） in MDSCs was examined by using RT-PCR, Western blot and immunofluorescent stain. It was found that one day after TGF-β1 treatment, the expression of CTGF, COL- I and COL-Ⅲ was increased dramatically. CTGF expression reached the peak on the day 2, and then decreased rapidly to a level of control group on the day 5. COL- I and COL-Ⅲ mRNA levels were overexpresed on the day 2 and 3 respectively, while their protein expression levels were up-regulated on the day 2 and reached the peak on the day 7. In group C, anti-CTGF could partly suppress the overexpression of COL-I and COL-Ill induced by TGF-131 one day after adding CTGF antibody. It was concluded that TGF-β1 could induce MDSCs to express CTGF, and promote the production of COL- I and COL-III. In contrast, CTGF antibody could partially inhibit the effect of TGF-β1 on the MDSCs by reducing the expression of COL- I and COL-III. Taken together, we demonstrated that TGF-β1-CTGF signaling played a crucial role in MDSCs synthesizing collagen proteins in vitro, which provided theoretical basis for exploring the methods postponing skeletal muscle fibrosis after nerve injury.The aim of this study was to investigate the mechanism of deposition of extracellular matrix induced by TGF-β1 in skeletal muscle-derived stem cells （MDSCs）. Rat skeletal MDSCs were obtained by using preplate technique, and divided into four groups： group A （control group）, group B （treated with TGF-β1, 10 ng/rnL）, group C （treated with TGF-β1 and anti-connective tissue growth factor （CTGF）, both in 10 ng/mL）, and group D （treated with anti-CTGF, 10 ng/mL）. The expression of CTGF, collagen type- I （COL- I ） and collagen type-III （COL-III） in MDSCs was examined by using RT-PCR, Western blot and immunofluorescent stain. It was found that one day after TGF-β1 treatment, the expression of CTGF, COL- I and COL-Ⅲ was increased dramatically. CTGF expression reached the peak on the day 2, and then decreased rapidly to a level of control group on the day 5. COL- I and COL-Ⅲ mRNA levels were overexpresed on the day 2 and 3 respectively, while their protein expression levels were up-regulated on the day 2 and reached the peak on the day 7. In group C, anti-CTGF could partly suppress the overexpression of COL-I and COL-Ill induced by TGF-131 one day after adding CTGF antibody. It was concluded that TGF-β1 could induce MDSCs to express CTGF, and promote the production of COL- I and COL-III. In contrast, CTGF antibody could partially inhibit the effect of TGF-β1 on the MDSCs by reducing the expression of COL- I and COL-III. Taken together, we demonstrated that TGF-β1-CTGF signaling played a crucial role in MDSCs synthesizing collagen proteins in vitro, which provided theoretical basis for exploring the methods postponing skeletal muscle fibrosis after nerve injury.

关 键 词：TGF-Β1 CTGF muscle-derived stem cells 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]