A Co-Dominant Marker BoE332 Applied to Marker-Assisted Selection of Homozygous Male-Sterile Plants in Cabbage (Brassica oleracea var.capitata L.)  

作　　者：CHEN Chen ZHUANG Mu FANG Zhi-yuan WANG Qing-biao ZHANG Yang-yong LIU Yu-mei YANG Li-mei CHENG Fei 

机构地区：[1]Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences [2]Horticulture College of Qingdao Agricultural University

出　　处：《Journal of Integrative Agriculture》2013年第4期596-602,共7页农业科学学报（英文版）

基　　金：supported by the National Science and Technology Ministry of China (2008BADB1B02 and 2009BADB8B03);the Core Research Budget of the Non-profit Governmental Research Institution (ICS, CAAS) (1610032011011);the China Agriculture Research System (CARS-25);the National High Technology Research and Development Program of China (863 Program, 2012AA100101)

摘　　要：The dominant genic male sterility （DGMS） gene CDMs399-3 derived from a spontaneous mutation in the line 79-399-3 of spring cabbage （Brassica oleracea var. capitata L.）, has been successfully applied in hybrid seed production of several cabbage cultivars in China. During the development of dominant male sterility lines in cabbage, the conventional identification of homozygous male-sterile plants （CDMs399-3/CDMs399-3） is a laborious and time-consuming process. For marker-assisted selection （MAS） of the gene CDMs399-3 transferred into key spring cabbage line 397, expressed sequence tag-simple sequence repeats （EST-SSR） and SSR technology were used to identify markers that were linked to CDMs399-3 based on method of bulked segregant analysis （BSA）. By screening a set of 978 EST-SSRs and 395 SSRs, a marker BoE332 linked to the CDMs399-3 at a distance of 3.6 cM in the genetic background of cabbage line 397 were identified. 7 homozygons male-sterile plants in population P1170 with 20 plants were obtained finally via MAS of BoE332. Thus, BoE332 will greatly facilitate the transferring of the gene CDMs399-3 into the key spring cabbage line 397 and improve the application of DGMS in cabbage hybrid breeding.The dominant genic male sterility （DGMS） gene CDMs399-3 derived from a spontaneous mutation in the line 79-399-3 of spring cabbage （Brassica oleracea var. capitata L.）, has been successfully applied in hybrid seed production of several cabbage cultivars in China. During the development of dominant male sterility lines in cabbage, the conventional identification of homozygous male-sterile plants （CDMs399-3/CDMs399-3） is a laborious and time-consuming process. For marker-assisted selection （MAS） of the gene CDMs399-3 transferred into key spring cabbage line 397, expressed sequence tag-simple sequence repeats （EST-SSR） and SSR technology were used to identify markers that were linked to CDMs399-3 based on method of bulked segregant analysis （BSA）. By screening a set of 978 EST-SSRs and 395 SSRs, a marker BoE332 linked to the CDMs399-3 at a distance of 3.6 cM in the genetic background of cabbage line 397 were identified. 7 homozygons male-sterile plants in population P1170 with 20 plants were obtained finally via MAS of BoE332. Thus, BoE332 will greatly facilitate the transferring of the gene CDMs399-3 into the key spring cabbage line 397 and improve the application of DGMS in cabbage hybrid breeding.

关 键 词：cabbage （Brassica oleracea var. capitata L.） dominant genic male sterility （DGMS） expressed sequencetag-simple sequence repeats （EST-SSR） bulked segregant analysis （BSA） marker-assisted selection （MAS） 

分 类 号：S634.3[农业科学—蔬菜学]