Conophylline Promotes the Proliferation of Immortalized Mesenchymal Stem Cells Derived from Fetal Porcine Pancreas (iPMSCs)  

作　　者：ZHANG Hui-ru LI Dan CAO Hui Lü Xiao CHU Yuan-kui BAI Yao-fu JIN Ya-ping PENG Sha DOU Zhong-ying HUA Jin-lian 

机构地区：[1]College of Bioengineering, Henan University of Technology [2]College of Veterinary Medicine, Shaanxi Center of Stem Cells Engineering & Technology/Key Lab for Animal Biotechnology of Agriculture Ministry of China, Northwest A&F University

出　　处：《Journal of Integrative Agriculture》2013年第4期678-686,共9页农业科学学报（英文版）

基　　金：supported by the grants from the National Natural Science Foundation of China(31272518, 31101775, 30972097);the Doctoral Fund of Ministry of Education of China (20100204120020);the Program for New Century Excellent Talents of State Ministry of Education (NCET-09-0654);the Scientific Research Program of Shaanxi Province (2011K02-06, 2008K02-05);the Scientific Research Program of Shaanxi Province, China(2011K02-06);the Fundamental Research Funds for the Central Universities, China (QN2011012);the Graduate Education Innovation Projects of Henan University of Technology, China (11YJCX45)

摘　　要：Conophylline, is a bis （indole） alkaloid consisting of two pentacyclic aspidosperma skeletons, isolated from Tabernaemontana divaricata, which has been found to induce β-cell differentiation in rat pancreatic acinar carcinoma cells and in cultured rat pancreatic tissue. However, the precise role of conophylline in the growth and survival of immortalized pancreatic mesenchymal stem cells （iPMSCs） derived from fetal porcine pancreas were not understood at present. To determine whether this molecule is involved in controlling the proliferation of iPMSCs, we examined the effects of conophylline on iPMSCs. We found that conophylline can robustly stimulate iPMSCs proliferation, even promote their potential differentiation into islet-like clusters analyzed by cell counting, morphology, RT-PCR and real-time PCR, Western blotting, glucose-stimulated insulin release and insulin content analysis. The effects of conophylline were inhibited by LY294002, which is the inhibitor of the PI3K pathway. These results suggest that conophylline plays a key role in the regulation of cell mass proliferation, maintenance of the undifferentiated state of iPMSCs and also promotes iPMSCs differentiated into insulin-producing cells.Conophylline, is a bis （indole） alkaloid consisting of two pentacyclic aspidosperma skeletons, isolated from Tabernaemontana divaricata, which has been found to induce β-cell differentiation in rat pancreatic acinar carcinoma cells and in cultured rat pancreatic tissue. However, the precise role of conophylline in the growth and survival of immortalized pancreatic mesenchymal stem cells （iPMSCs） derived from fetal porcine pancreas were not understood at present. To determine whether this molecule is involved in controlling the proliferation of iPMSCs, we examined the effects of conophylline on iPMSCs. We found that conophylline can robustly stimulate iPMSCs proliferation, even promote their potential differentiation into islet-like clusters analyzed by cell counting, morphology, RT-PCR and real-time PCR, Western blotting, glucose-stimulated insulin release and insulin content analysis. The effects of conophylline were inhibited by LY294002, which is the inhibitor of the PI3K pathway. These results suggest that conophylline plays a key role in the regulation of cell mass proliferation, maintenance of the undifferentiated state of iPMSCs and also promotes iPMSCs differentiated into insulin-producing cells.

关 键 词：immortalized pancreatic mesenchymal stem cells （iPMSCs） conophylline PI3K insulin-producing cells 

分 类 号：S828[农业科学—畜牧学]