对二氯苯对湿地土壤细菌群落多样性的影响  

Effect of 1,4-Dichlorobenzene on Diversity of Bacteria Communities in Wetland Soil

作　　者：徐文品[1,2] 丁成[1,2] 杨春生[1,2] 朱光灿 杨唐仪[1,2] 

机构地区：[1]江苏大学环境学院,江苏镇江212013 [2]盐城工学院化学与生物工程学院,江苏盐城224051 [3]江苏省盐城市环境保护检测中心站,江苏盐城224051

出　　处：《湖北农业科学》2012年第18期4134-4137,共4页Hubei Agricultural Sciences

基　　金：江苏省自然科学基金项目(BK2009171);江苏省高校"青蓝工程"基金项目

摘　　要：利用聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)考查了对二氯苯胁迫对湿地土壤细菌群落多样性的影响。通过不同培养时间(4、18和45d)和不同培养浓度(120、240、360和600mg/kg)对二氯苯污染下湿地土壤细菌群落基因组的变性梯度凝胶电泳(DGGE)多样性分析。结果表明,各处理下的土壤细菌群落多样性在不同处理时间有明显差异。培养初期对二氯苯对土壤细菌群落抑制作用非常显著,土壤细菌中某些群落的生长受到抑制;不同处理在处理后期差异很小。在土壤培养初期,含对二氯苯240mg/kg的培养基上出现一条新增条带,为对照和低浓度对二氯苯土壤所没有,说明土壤中存在对二氯苯生长优势菌。The effect of different concentration of 1,4-dichlorobenzene（120,240,360 and 600 mg/kg） with different cultivation time（4,18 and 45 d） on wetland bacterial community diversity was studied using PCR-DGGE technique.The results showed that the effect of each concentration of 1,4-dichlorobenzene on genetic diversity of soil bacteria differed at different treating time.In early treatment period,soil bacterial community was inhibited obviously.The difference among different treatments in later period was little.In early treatment period,a new medium band emerged in the culture medium of 240 mg/kg of 1,4-dichlorobenzene treatment,indicating that there were special microbial species resistant to 1,4-dichlorobenzene existed in the soil.

关 键 词：变性梯度凝胶电泳(DGGE) 对二氯苯 湿地土壤细菌菌群 多样性 

分 类 号：Q935[生物学—微生物学]