锌指核酸酶技术在动物转基因中的应用  

Application of Zinc Finger Nuclease Technology in Transgenic Animals

作　　者：王刘豪[1] 王吉田[1] 余昊[1] 王运兵[1] 

机构地区：[1]河南科技学院资源与环境学院,河南新乡453000

出　　处：《湖北农业科学》2012年第19期4177-4180,共4页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(31101497)

摘　　要：锌指核酸酶(Zinc finger nuclease,ZFN)具有特异性识别并敲除DNA片段中特定基因的特点,通过对DNA片段上的特定基因进行靶向修饰产生新型细胞,是能够应用于动物转基因上的一种新技术。该技术已在一些动物的研究上取得了一定的成果,相对于传统的转基因技术,该技术在简化操作程序、缩短操作时间及提升成功率上都有很大的提高。就常用的动物转基因方法、锌指核酸酶技术的作用原理以及在动物转基因上的应用及其前景进行了论述。Zinc finger nuclease（ZFN） has the characteristics of identifying and knocking out specific genes in DNA fragments.New cells could be produced through a specific gene targeted modification in a specific DNA fragment.It can be used as a new technology in transgenic animals.Some achievements of this technology have been made in research on some animals.Compared with traditional transgenic technology,this technology could greatly simplify operation procedures,shorten operation time and improve successful rate.Commonly used methods in transgenic animals,principles of zinc finger nuclease technology and application prospects of the technology were discussed in the paper.

关 键 词：锌指核酸酶(Zinc FINGER nuclease ZFN) 动物转基因 靶向修饰 

分 类 号：Q789[生物学—分子生物学]