大白菜再生体系的正交设计优化  

Establishment of Regeneration System of Chinese Cabbage with Orthogonal Design

作　　者：吴玲玲[1] 王一娟 董刚[1] 陈竹君[2] 裴雁曦[1] 

机构地区：[1]山西大学生命科学学院,山西太原030006 [2]浙江大学园艺系,浙江杭州310029

出　　处：《山西大学学报（自然科学版）》2012年第4期712-716,共5页Journal of Shanxi University(Natural Science Edition)

基　　金：国家自然科学基金(31071809);教育部博士点(博导类)项目(20091401110004);山西省优秀青年学术带头人支持计划(200906)

摘　　要：以山西地区广泛栽培的5个大白菜品种为材料,带柄子叶为外植体,通过正交设计试验研究了品种、NAA、6-BA、AgNO3等因素对大白菜离体再生的影响.结果显示:品种津育75在培养基MS+2mg/L 6-BA+0.3mg/L NAA+7mg/L AgNO3的条件下再生率最高,达76.92%.Five varieties of Chinese cabbage（Brassica campestris ssp.pekinensis） widely cultivated in Shanxi were used as materials and cotyledon with petioles as explants.The effects of NAA,varieties,6-BA,AgNO 3 on regeneration of Chinese cabbage in vitro were studied by orthogonal design.The results showed that Jinyu 75had the highest regeneration frequency,which was 76.92%in the MS medium containing 2mg / L 6-BA ＋0.3mg / L NAA ＋7mg / L AgNO 3.

关 键 词：大白菜 外植体 正交设计 再生率 

分 类 号：TS255.2[轻工技术与工程—农产品加工及贮藏工程]