五味子乙素对染矽尘大鼠肺组织MMP-2和TIMP-2蛋白表达的动态影响  

Effect of Schisandrin B on Dynamic Change of MMP-2 and TIMP-2 Expression in Rat Lungs Exposed to Silica

作　　者：郭民[1] 樊林花[1] 陈朝阳[1] 刘田福[1] 

机构地区：[1]山西医科大学实验动物中心,山西太原030001

出　　处：《山西大学学报（自然科学版）》2012年第4期726-730,共5页Journal of Shanxi University(Natural Science Edition)

基　　金：山西省实验动物专项基金项目(2007k03);山西医科大学2011年大学生创新基金项目

摘　　要：为研究五味子乙素(Sch-B)对二氧化硅致大鼠矽肺中基质金属蛋白酶(MMP-2)和其抑制物基质金属蛋白酶组织抑制因子(TIMP-2)的作用,采用暴露式气管内注入SiO2混悬液法建立大鼠矽肺模型,染毒后1d开始灌胃给予Sch-B干预治疗,药物干预3d、7d、14d和28d后处死取其肺组织.应用HE、Masson染色法观察肺组织病理改变及肺组织胶原纤维变化;通过ELISA法对大鼠肺组织中不同时间点的MMP-2、TIMP-2蛋白的表达情况进行了研究.结果表明:Sch-B对矽肺组织纤维化病变有明显的改善,胶原纤维明显减少.Sch-B对二氧化硅致大鼠矽肺中不同时间点肺组织MMP-2的表达均有一定程度的抑制作用,早期肺泡炎时可一过性提高TIMP-2的表达,起到抑制MMP-2对肺组织损伤的作用,后期抑制TIMP-2的表达,减缓肺组织纤维化的发生.To investigate the effect of schisandrin B on expression and significance of matrix metalloproteinase（MMP-2） and tissue inhibitor of matrix metall oproteinase（TIMP-2） protein in silica induced pulmonary fibrosis model,the lung injury model was established by a single intratracheal injection of silica.From the first day of exposure of silica,the rats were treated with Sch-B and sacrificed on 3,7,14 and 28 d and lungs were collected.The histopathological of the lungs were observed by HE staining and changs of collagen fibers in the lungs were observed by Masson staining.The MMP-2 and TIMP-2 protein expression were determined by ELISA.The results show that compared with control group,Sch-B improved fibrosis of lungs and reduced collagen fibers.Sch-B could inhibit expression of MMP-2 protein in model rats at every time points,and improve expression of TIMP-2 at the initial stage of rat lungs exposed to silica.Sch-B can inhibit MMP-2 induced lung injury.Sch-B inhibited expression of TIMP-2 at the final stage and reduced pulmonary fibrosis.

关 键 词：五味子乙素 二氧化硅矽肺 MMP-2、TIMP-2 大鼠 

分 类 号：R332[医药卫生—人体生理学] R135.2[医药卫生—基础医学]