大麻脱胶功能菌株在沤麻中的应用  

Application of Functional Degumming Bacteria in Hemp Retting

作　　者：于文杰[1,2] 王贵宾[1,2] 韩洁[1,2] 接伟光[3] 葛菁萍[1,2] 蔡柏岩[1,2,3] 

机构地区：[1]黑龙江大学生命科学学院黑龙江省普通高等学校微生物学重点实验室,哈尔滨150080 [2]农业微生物技术教育部工程研究中心,哈尔滨150500 [3]黑龙江东方学院食品与环境工程学部,哈尔滨150086

出　　处：《湖北农业科学》2012年第21期4772-4775,共4页Hubei Agricultural Sciences

基　　金：哈尔滨市科技攻关计划项目(2007AA6CN032);黑龙江省教育厅面上项目(11531271);黑龙江省博士后启动基金(LBH-Q05113);黑龙江省自然科学基金项目(C200505);黑龙江省人事厅留学回国择优资助项目;黑龙江省教育厅振兴老工业基地成果转化项目(1152GZH03)

摘　　要：将实验室自行分离的功能菌株HDDMM02、HDDMG05培养至最大产酶时期后用于大麻(Cannabissativa)脱胶沤麻,并对大麻纤维化学成分进行测定。结果显示两种菌株最佳的接种量为麻原料体积的0.5%,尿素和NaCl的最佳添加量均为1.0 g/L。在此条件下进行中试,对麻纤维各项指标进行检测。结果表明加菌沤制麻纤维的平均出麻率比正常沤制时增加了1.45个百分点,平均强度提高了23.67 N,断裂比强度提高了0.21 cN/dtex,麻束有效强度伸长了0.16 m。扫描电镜图片显示加菌沤麻纤维脱胶完全且粘附在纤维表面,单纤维已基本游离出来。Functional hemp degumming strains HDDMM02 and HDDMG05 isolated in laboratory were cultured to maximum enzyme production phase and inoculated for degumming and retting of Cannabis sativa fiber.The chemical composition of hemp fiber was determined;and it was showed that the optimum inoculation dose of the 2 strains was 0.5% of the volume of raw hemp;the optimal dose of urea and NaCl was 1.0 g/L.Pilot test was conducted under these conditions and indexes of hemp fiber was measured.The result showed that adding retting bacteria could increase hemp fiber yield by 12.44%,enhance hemp fiber strength by 23.67 N,improve fiber breakage strength by 0.21 cN/dtex,and increase hemp-fiber-bundle effective length by 0.16 cm.Compared to CK,SEM images showed the gum could be completely removed from the hemp with the bacteria adhered to the fiber surface;and single fiber was nearly dissociated in treatment adding degumming fiber.

关 键 词：大麻(Cannabis sativa) 脱胶功能菌株 沤麻 

分 类 号：S563.3[农业科学—作物学]