基于SRAP-PCR分析的百合鳞片DNA提取  

DNA Extraction from Squama Leaves of Liliwn lancifolium for SRAP-PCR Analysis

作　　者：李家敏[1] 周秀玲[1] 

机构地区：[1]宜春学院化学与生物工程学院,江西宜春336000

出　　处：《湖北农业科学》2012年第21期4896-4898,共3页Hubei Agricultural Sciences

基　　金：江西省宜春市科技计划项目(JXYC2009KNA007)

摘　　要：采用改良的CTAB法从百合(Liliwn lancifolium Thunb.)鳞片中提取DNA,采用紫外分光光度法和琼脂糖凝胶电泳检测所提取的DNA质量,并进行SRAP-PCR分析。结果表明,改良的CTAB法提取的百合DNA A260 nm/A280 nm为1.69,浓度为0.30μg/μL。琼脂糖胶电泳检测结果显示提取的DNA为清晰的条带,RNA去除干净,无降解现象。SRAP-PCR扩增产物多态性好,稳定性高,可用于遗传多样性分析。Modified CTAB method was applied to extract DNA from squama leaves of Liliwn lancifolium Thunb..Ultraviolet spectrophotometry and agarose gel electrophoresis as well as SRAP-PCR were adopted to test the quality of extracted DNA.The results showed that A260 nm/A280 nm of DNA was 1.69,and concentration was 0.30 μg/μL.The agarose gel electrophoresis showed that the DNA had clear band without degradation or RNA.The SRAP-PCR product was with good polymorphism and stability,which could be used for genetic polymorphism analysis of Liliwn.

关 键 词：百合(Liliwn) 鳞片 SRAP DNA提取 

分 类 号：Q523[生物学—生物化学]