磷酸化油菜秸秆纤维素吸附剂的制备和对牛血清白蛋白的吸附  

Preparation of Phosphorylated Cole Straw and Its Adsorption Behavior for BSA

作　　者：李步海[1] 石荧原[1] 

机构地区：[1]中南民族大学化学与材料科学学院分析化学国家民委重点实验室,武汉430074

出　　处：《中南民族大学学报（自然科学版）》2013年第1期1-6,共6页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：国家民委院校科研基金资助项目(MJY00004)

摘　　要：以天然产物油菜秸秆纤维素粉为基质,二甲基甲酰胺为交联剂,磷酸为修饰剂,制备了新型磷酸化油菜秸秆纤维素生物吸附剂.用红外光谱、透射电子显微镜和X射线光电子能谱,对油菜秸秆纤维素粉和磷酸化油菜秸秆纤维素吸附剂进行了表征.研究了油菜秸秆纤维素粉改性前后溶液的pH、吸附时间、BSA的初始浓度、温度和离子强度等因素对牛血清白蛋白(BSA)的吸附的影响.结果表明:在25℃,pH 5.0、6.0,吸附20 h的条件下,磷酸化油菜秸秆纤维素粉微球对BSA的吸附容量为127.39 mg/g,而未修饰油菜秸秆纤维素粉微球对BSA的吸附容量只有68.98 mg/g,说明改性获得较好的性能.吸附剂的吸附等温线符合Langmuir方程,属单分子层吸附.A novel biosorbent was prepared from cole straw using dimethyl formamide and phosphoric acid as crosslinker and modification agents repectively.The absorbents were characterized by FTIR,TEM and XPS.Such affecting factors as pH,adsorption time,initial BSA concentration,temperature and NaCl ionic strength before and after the modification of cole straw were extensively investigated.The results showed that the maximum adsorption capacity of the phosphorylated cole straw was 127.39 mg/g at pH 5.0、6.0,25 ℃ for 20 h,obtaining better performance compared to the 68.98 mg/g from unmodified materials.The adsorption phenomena were found monolayer adsorption following Langmuir isotherm.

关 键 词：油菜秸秆纤维素 磷酸化 吸附 牛血清白蛋白 

分 类 号：O652.6[理学—分析化学]