东方田鼠IgG和IgG_3对日本血吸虫抗原反应特性的研究  

NON-SPECIFIC REACTIVITIES OF IgG AND IgG_3 FROM HEALTHY MICROTUS FORTIS WITH SCCHISTOSOMA JAPONICUM ANTIGENS

作　　者：李浩[1] 刘金明[1] 傅志强[1] 石耀军[1] 金亚美[1] 陆珂[1] 姚利晓[1] 杨健美[1] 林矫矫[1] 苑纯秀[1] 朱传刚[1] 洪炀[1] 

机构地区：[1]中国农业科学院上海兽医研究所国家防治动物血吸虫病专业实验室农业部动物寄生虫学重点开放实验室,上海200241

出　　处：《中国动物传染病学报》2012年第4期74-78,共5页Chinese Journal of Animal Infectious Diseases

基　　金：上海市科学技术委员会科研计划项目(054909002);国家自然科学基金项目(31072129)

摘　　要：本文对东方田鼠(Microtus fortis)总IgG和IgG3对日本血吸虫抗原的反应特性进行了研究。纯化东方田鼠IgG,用间接ELISA法检测不同浓度东方田鼠总IgG和IgG3对日本血吸虫童虫可溶性抗原(soluble schistosomula antigen,SSA)、成虫可溶性抗原(soluble adult worm antigen,SWA)、虫卵可溶性抗原(soluble egg antigen,SEA)、童虫不可溶性抗原(insoluble schistosomula antigen,ISSA)、雄虫不可溶性抗原(insoluble male worm antigen,ISMA)、雌虫不可溶性抗原(insoluble female worm antigen,ISFA),以及基因工程抗原rSj14、rSj23-HLD、rSJ28GST、rSjPP的反应情况。结果显示,HRP标记免抗东方田鼠IgG抗体和羊抗小鼠IgG3抗体作为二抗,A值变化规律一致。该结果显示东方田鼠总IgG和IgG3与日本血吸虫抗原可能存在非特异性反应。Total IgG and IgG3 were prepared from healthy Microtus fortis （M. fortis） and used in indirect ELISA to examine their reactivity profiles with antigens of Schistosoma japonicum at different stages. The antigens used in the study included soluble schistosomula antigen （SSA）, soluble adult worm antigen （SWA）, soluble egg antigen （SEA）, insoluble schistosomula antigen （ISSA）, insoluble male worm antigen （ISMA）, insoluble female worm antigen （ISFA） and recombinant antigens of rSj 14, rSj23-HLD, rSJ28GST and rSjPP. The results showed that the change of A votue was similar for HRP-conjugated Rabbit anti-M.fortis or goat anti-mouse as second antibody. These results revealed that antibodies ofM. foms might nonspecifically reacted with S. japonicum antigens.

关 键 词：东方田鼠 日本血吸虫 IGG ELISA 

分 类 号：S852.735[农业科学—基础兽医学]