Validation of Monomeric Anthocianin Determination Method for Bilberry Juice and Marc Extracts  

作　　者：Ance Dandena Ieva Leimane Ugis Kletnieks 

机构地区：[1]Silvanols Ltd., Kurbada iela 2, Riga LV-1009, Latvia

出　　处：《Journal of Life Sciences》2012年第12期1378-1382,共5页生命科学（英文版）

摘　　要：The solid waste generated in industrial berry juice production was considered as a low cost raw material for the extraction of natural antioxidants. Berries contain phenolic compounds with high antioxidant potential, including anthocianin. Quantitative determination method for monomeric anthocianins in bilberry juice and marc was validated. An official method from Association of Analytical Communities was used to determine anthocianins in juice and marc extracts by measuring light absorption for solutions with pH values 1.0 and 4.5 at 520 nm and 700 rim. Results were expressed as cyanidin-3-glucoside equivalent, as it is the most common anthocianin pigment. Calibration curve was obtained, linearity was checked, working interval and accuracy was determined and samples were tested. Mentioned method was evaluated as appropriate for quantitative anthocianin analysis in bilberry juice and marc. Necessity of calibration curve was approved using extinction coefficient of cyanidin-3-glucoside instead. Method assures adequate precision and accuracy as well.

关 键 词：Natural antioxidants monomeric anthocianins bilberry juice bilberry marc. 

分 类 号：S859.84[农业科学—临床兽医学] TS202.3[农业科学—兽医学]