HPLC测定凤眼草中槲皮素与山柰素的含量  

HPLC Determination of Quercetin and Kaempferol in Ailanthi Fructus

作　　者：孟宪波 

机构地区：[1]淄博市药品检验所,山东淄博255086

出　　处：《中国实验方剂学杂志》2013年第8期77-79,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立以高效液相色谱法测定凤眼草中槲皮素与山柰素含量的方法。方法:Inertsil ODS-3色谱柱(4.6mm×250 mm,5μm),流动相甲醇-0.4%磷酸溶液(50∶50),检测波长360 nm,流速1.0 mL·min-1。结果:槲皮素在11.1～333μg(r=1)线性关系良好,平均回收率为97.82%,RSD 1.1%;山柰素在2.3～69μg(r=1)线性关系良好,平均回收率为96.81%,RSD 1.7%。结论:方法简便、快速、重复性好,可用于凤眼草的质量控制。Objective：To establish an HPLC method for the simultaneous determination of quercetin and kaempferol in Ailanthi Fructus.Method：Quercetin and kaempferol were separated on Inertsil ODS-3（4.6 mm×250 mm,5 μm） column and detected at 360 nm.The mobile phase was methanol-0.4% phosphate（50∶50）.The flow rate was 1.0 mL·min-1.Result：Quercetin and kaempferol were linear within the range of 11.1-333 μg（r=1）,2.3-69 μg（r=1） respectively.The average recovery was 97.82% with RSD of 1.1%,96.81% with RSD of 1.7% respectively.Conclusion：The method is simple,reproducible,and it can be used for the quality control of Ailanthi Fructus.

关 键 词：凤眼草 槲皮素 山柰素 含量测定 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]