不同纯化方法对金银花提取物中咖啡酰奎宁酸类成分含量的影响  

Effects of Different Purification Methods on Content of Caffeoyl Quinic Acids from Flos Lonicerae Extractive

作　　者：邱新建 赵海鹏 刘向东 苏瑞强 李守信 赵志全 

机构地区：[1]鲁南制药集团股份有限公司,山东临沂276006 [2]中药制药共性技术国家重点实验室,山东临沂276006 [3]中药制药新技术山东省企业重点实验室,山东临沂276006

出　　处：《中国实验方剂学杂志》2013年第8期83-86,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重点基础研究发展计划(973计划)项目(2012CB724001)

摘　　要：目的:考察不同纯化方法对金银花提取物中咖啡酰奎宁酸类成分的影响。方法:应用醇沉法、石硫法、石硫醇法、大孔树脂吸附法及萃取法等对金银花进行提取纯化,并采用高效液相色谱法测定其中6种咖啡酰奎宁酸类成分的含量。结果:萃取法所得金银花提取物中咖啡酰奎宁酸类成分的含量最高,总咖啡酰奎宁酸含量达83.91%。结论:萃取法是制备金银花提取物的理想方法。Objective：To study the effects of different purification ways on the extractive rate of caffeoyl quinic acids in Flos Lonicerae extractive.Method：Several purification processes were used respectively in the preparation of Flos Lonicerae extractive,such as alcohol precipitation method,lime-sulfur method,lime-sulfur ethanol method,macroporous resin method,and extraction method etc.The content of the six caffeoyl quinic acids was determined by HPLC.Result：The content of the caffeoyl quinic acids in Flos Lonicerae extractive by extraction method was higher than the other methods.Total content of caffeoyl quinic acids was up to 83.91%.Conclusion：The extraction method is the best in the preparation of Flos Lonicerae extractive.

关 键 词：金银花 咖啡酰奎宁酸 绿原酸 萃取法 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]