HPLC同时测定不同产地葛根中6种主要异黄酮类成分的含量  

Simultaneous Determination of Six Isoflavonoids in Radix Puerariae Lobatae from Different Habitats by HPLC

作　　者：王治平[1] 李卫民[2] 高英[2] 刘杰[2] 

机构地区：[1]广东药学院,广州510006 [2]广州中医药大学,广州510006

出　　处：《中国实验方剂学杂志》2013年第8期125-128,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立同时测定葛根药材中6种主要异黄酮类成分含量的HPLC方法。方法:采用RP-HPLC法,KromasilC18(4.6 mm×250 mm,5μm)柱;流动相:甲醇-0.1%枸橼酸溶液,梯度洗脱;流速1.0 mL·min-1,柱温30℃,检测波长250 nm。结果:3’-羟基葛根素、葛根素、大豆苷、染料木苷、芒柄花苷、大豆苷元的线性范围分别为76.2～1 016,65.8～1 316,85.6～1712,49.98～499.8,21.94～219.4,48.5～485 ng,相关系数(R2)均大于0.999 6,6种成分的平均加样回收率分别为99.73%(RSD 0.6%),100.48%(RSD 1.11%),100.37%(RSD 0.79%),100.28%(RSD 1.07%),99.96%(RSD 1.22%),97.36%(RSD 1.59%)。结论:方法简便快速,重复性良好,结果准确可靠,可用于葛根药材中6种主要异黄酮类成分的含量测定。Objective： A reversed-phase high performance liquid chromatographic method （RP-HPLC） was developed for the simultaneous analysis of six isoflavonoids of Gegen, the roots of Puerariae lobatae. Method： HPLC determination was performed on a Kromasil C18 column （4.6 mm×250 mm,5μm） and detected at 250 nm. The mobile phase was consisted of methanol and 0.1% citric acid solution with gradient elution. The flow rate was 1.0 mL .min-1, and column temperature was kept at 30℃. Result： The method was proved to be linear in the ranges of 76.2-1 016, 65.8-1 316, 85.6-1 712, 49.98-499.8, 21.94-219.4, 48.5-485 ng for the six isoflavonoid, 3＇-hydroxypuerarin, puerarin, daidzin, genistin, ononin, daidzein, respectively. The averagerecoveries were 99.73% （RSD 0.6%）, 100.48% （RSD 1.11%）, 100.37% （RSD 0.79%）, 100.28% （RSD 1.07%）, 99.96% （RSD 1.22%）, 97.36% （RSD 1.59%）, respectively. Conclusion： Results obtained showed that the method is convenient, accurate and reliable, can be applied to the simultaneous determination of the six isoflavonoids in Radix Puerariae lobatae.

关 键 词：高效液相色谱 葛根异黄酮 3’-羟基葛根素 葛根素 大豆苷 染料木苷 大豆苷元 芒柄花苷 

分 类 号：R284.1[医药卫生—中药学]