补肾方胚胎期干预子代成年大鼠IGT及其胰岛素抵抗、瘦素抵抗的研究  

Study on Preventive Effect of Prescription for Tonifying Kidney During Embryonic Period on IGT and Insulin Resistance,Leptin Resistance

作　　者：许凯霞[1] 王永辉[1] 杨向竹[2] 牛欣[2] 冯前进[1] 

机构地区：[1]山西中医学院,太原030024 [2]北京中医药大学,北京100029

出　　处：《中国实验方剂学杂志》2013年第8期167-171,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技部国际科技合作项目(2006DFA32490);山西省科技基础条件平台建设项目(2009091010)

摘　　要：目的:通过大鼠胚胎发育期予补肾方干预,以研究中医补肾方在胚胎发育期干预能否预防高脂饮食诱发的子代成年大鼠糖耐量低减(IGT)及其对胰岛素、瘦素的影响。方法:将孕鼠分为正常对照组、模型组、左归丸组、右归丸组、八珍汤组,在孕1～19 d,各用药组分别以左归丸、右归丸、八珍汤灌胃(ig,20 g·kg-1,20 mL·kg-1),正常对照组和模型组以生理盐水灌胃。除正常对照组外,其余各组子鼠均于6周龄开始高脂饲料喂养,12周后测各组子鼠空腹血糖(FBG)、糖负荷后2 h血糖(2hBG)、血清空腹胰岛素、瘦素。结果:与模型组比较,左归丸组2hBG明显降低(P<0.05);八珍汤组和右归丸组2hBG无明显降低。左归丸组、右归丸组、八珍汤组胰岛素抵抗指数(HOMA-IR)均较模型组显著降低(P<0.01,P<0.01,P<0.05)。左归丸组和右归丸组血清瘦素显著低于模型组(P<0.01,P<0.01);而八珍汤组血清瘦素水平与模型组相比无显著性差异。结论:左归丸与右归丸胚胎期干预都可对抗高脂饮食引发子代成年大鼠胰岛素抵抗、瘦素抵抗,但对IGT的预防作用以左归丸为优。八珍汤组对抗胰岛素抵抗、瘦素抵抗作用相对弱,也未能预防高脂饮食引起的子鼠IGT的发生。Objective：This project aimed to find if prescription for tonifying kidney fed during embryonic development can prevent impaired glucose tolerance（IGT） caused by feeding high fat diet and its influence on insulin and leptin.Method：The pregnant rats were randomly divided into 5 groups.The normal control group and model group were fed by normal saline on the 1st day,ig,20 mL·kg-1.In other 3 experiment groups,the pregnant rats were fed respectively with Zuogui Wan（ZGW,a prescription for reinforcing kidney Yin） or Yougui Wan（YGW,a prescription for reinforcing kidney Yang）or Bazhen decoction（BZD,a prescription for supplementing Qi and Blood）,ig,20 g·kg-1,20 mL·kg-1,at the same time.After birth,the offsping rats in the other four groups except the control group were fed with high fat diet in 6th week.After 12 weeks of high fat diet,the fasting blood glucose（FBG）,2 h blood glucose（2 hBG）,fasting serum insulin and serum leptin concentrations were measured.Result：2 hBG of ZGW group was significant lower than 2 hBG of model group（P0.05）;2 hBG of ZGW group and YGW group was not significantly different from model group.HOMA-IR of ZGW group,YGW group and BZD group was significantly lower than HOMA-IR of model group（P0.01,P0.01,P0.05）.Leptin of ZGW group and YGW group was significantly lower than leptin of model group（P0.01,P0.01）.Leptin of BZD group was not significantly different from model group.Conclusion：ZGW and YGW during embryonic development can all withstand the adverse changes of serum insulin,and ZGW can prevent IGT caused by high fat diet,but YGW can not.BZD during embryonic development can also withstand the adverse changes of serum insulin and can not withstand the adverse changes of serum leptin,which is not as good as ZGW and YGW,and can not prevent IGT caused by high fat diet.

关 键 词：补肾方 胚胎期 子代大鼠 糖耐量低减 胰岛素 瘦素 

分 类 号：R285.5[医药卫生—中药学]