三七总皂苷对快速老化痴呆模型小鼠SAMP8炎症因子及小胶质细胞的影响  

Effect of Panax notoginseng Saponins on Inflammatory Factors and Microglia Cell of SAMP8 Mice

作　　者：黄金兰[1] 陆璐[2] 黄丹[2] 冯颖琴 崔雯[2] 张雯艳[2] 钟振国[1] 

机构地区：[1]广西医科大学,南宁530021 [2]广西中医药大学,南宁530001

出　　处：《中国实验方剂学杂志》2013年第8期171-174,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30960489;81160512);广西中医药大学中药药效研究重点实验室开放课题(09-007-06-07)

摘　　要：目的:研究三七总皂苷(PNS)对快速老化痴呆模型小鼠(SAMP8)炎症因子及小胶质细胞的影响。方法:采用SAMP8小鼠,将其随机分为模型组、PNS高剂量组(200 mg·kg-1)、PNS低剂量组(100 mg·kg-1)和石杉碱甲组(0.3 mg·kg-1),8只/组,模型组ig给予给药组相同容积的双蒸水。每天ig 1次,连续给药2个月。用ELISA双抗体夹心法法测定PNS对各组SAMP8小鼠血清中的细胞因子白介素(IL)-1β,IL-6、肿瘤坏死因子(TNF-α)及补体(C1q)表达的影响;HE染色光镜观察小胶质细胞的形态变化。结果:PNS高剂量组的IL-1β(23.14±12.58)ng·L-1水平低于模型组(47.44 ng·L-1)(P<0.05);IL-6 PNS高、低剂量组(243.3±133.9),(269.0±75.1)ng·L-1低于模型组(582.5±144.6)ng·L-1(P<0.05);模型组小鼠血清中补体C1q(280.4±49.75)mg·L-1含量均高于阳性组C1q(144.71±21.78)mg·L-1和PNS高剂量组C1q(187.7±65.56)mg·L-1;模型组小鼠海马区的小胶质细胞被激活,吞噬神经细胞;用药组海马区小胶质细胞没有被激活,为静息状态。血清中TNF-α各组间无显著差异。结论:PNS对SAMP8小鼠的免疫炎症反应有一定的抑制作用。Objective：To study the effect of Panax notoginseng saponins（PNS） on the inflammatory factors and microglia cell of senescence-accelerated mouse prone 8（SAMP8） mice.Method：SAMP8 mice were randomly divided into four groups：PNS high-dosage group,PNS low-dosage group,huperzine A group and model control group.The high-dosage group and low-dosage group were treated with 200,100 mg·kg-1 PNS respectively per day and the huperzine A group was treated with 0.3 mg·kg-1 huperzine A by intragastric administration,per day for 8 consecutive weeks.The serum cytokines of SAMP8 like interleukin（IL）-1β,IL-6,tumor necrosis factor-α（TNF-α）,complement 1q（C1q） were measured by ELISA.The morphological changes of microglia cells were observed by light microscopy with HE staining.Result：The level of IL-1β in the high-dose group was lower than that in the model group（P0.05）,and compared to the model group,the content of IL-6 in PNS high,low-dose groups showed significantly different（P0.05）.The level of C1q in the blank group was higher than that in the high-dose group and positive group.HE staining showed that microglia in the hippocampus of the model group was over-activation,and swallowed nerve cells,while the microglia in the treatment groups was not activated.Conclusion：PNS can reduce the inflammatory response in SAMP8 mice.

关 键 词：三七总皂苷 炎症因子 小胶质细胞 SAMP8 

分 类 号：R285.5[医药卫生—中药学]