高脂血症合并脑缺血对模型大鼠炎症因子的影响  

Hyperlipidemia Merger of Cerebral Ischemia in Rat Models of Inflammation Factor Influence

作　　者：张振强[1] 贾亚泉[1] 宋军营[1] 李澎涛[2] 潘彦舒[2] 王丛笑[1] 吕欢欢[1] 

机构地区：[1]河南中医学院,郑州450008 [2]北京中医药大学,北京100029

出　　处：《中国实验方剂学杂志》2013年第8期183-187,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技重大专项(2009ZX09502-014);河南省基础与前沿技术研究(122300410026)

摘　　要：目的:比较复合脑缺血模型和单纯脑缺血模型在不同时间炎症反应变化,分析高脂血症炎症因素对缺血脑组织的影响。方法:动物随机分为正常组、高脂对照组、假手术组、高脂假手术组、单纯缺血组和复合缺血组。取材时间是模型组脑缺血手术后3 d和7 d。采用高脂饲料饲养大鼠,复制经典的高脂血症动物模型,检测大鼠血清中血脂的含量以确定模型成功;复制大脑中动脉缺血模型,观察大鼠脑部缺血损伤情况。采用线栓法复制大鼠大脑中动脉缺血模型,观察大鼠脑部缺血损伤情况。用酶联免疫法(ELISA)检测血清中单核细胞趋化蛋白-1(monocyte chemotactic protein-1,MCP-1)、肿瘤坏死因子(tumor necrosis factor,TNF-α)T、C-反应蛋白(creactive protein,CRP)和超氧化物歧化酶(superoxide dismutase,SOD)的水平。结果:MCP-1,TNF-α和CRP在复合脑缺血组和单纯脑缺血组与相应假手术组组内比较,脑缺血组在缺血3,7 d血清含量均多于相应的假手术组,差异显著(P<0.05);复合脑缺血组与单纯脑缺血组组间比较,MCP-1,TNF-α在复合脑缺血组在缺血3,7d血清含量均增多,差异显著(P<0.05);CRP在复合脑缺血组织3 d血清含量均增多(P<0.05),缺血7 d血清含量均降低(P<0.05)。SOD在复合脑缺血组和单纯脑缺血组与相应假手术组组内比较,脑缺血组在缺血3,7 d活性均降低,差异显著(P<0.05);复合脑缺血组与单纯脑缺血组组间比较,复合脑缺血组在缺血3 d活性降低,差异显著(P<0.05)。结论:高脂血症条件下,炎症因子在缺血不同时期既有累积现象,又有特异性的表达,这可能在脑缺血恢复期有助于损伤的修复。Objective：To study the character of hyperlipidemia concurrent cerebral ischemia and analyze the influence of hyperlipidemia inflammation factor on cerebral ischemia by comparing inflammation change in different time change between hyperlipidemia and cerebral ischemia model and normal rat.Method：Dividing experimental rats into normal control,normal sham operation group,day 3,day 7 after normal ischemia and hyperlipid,sham operation group,day 3,day 7 after ischemia with hyperlipidemia.Based on time is day 3 or day 7 after cerebral ischemia.Building classic hyperlipidemia rat model with high fat diet,to confirm a successful model and detect the concentration of serum lipid.Constructing middle cerebral artery occlusion model,and observe the brain ischemia injury of rat.Detect the concentration of monocyte chemotactic protein-1（MCP-1）,tumor necrosis factor（TNF-α）,creactive protein（CRP） and superoxide dismutase（SOD） in serum between groups by ELISA.Result：The concentration of MCP-1,TNF-α and CRP were increased in day 3 and day 7 after cerebral ischemia model.Compared to normal cerebral ischemia group,in hyperlipidemia concurrent cerebral ischemia group,the concentration of MCP-1,TNF-α were higher with significant difference in day 3 and in day 7 after ischemia（P0.05）.The concentration of CRP were higher with significant difference in day 3 and were prominently decreased in day 7 after ischemia（P0.05）.The concentration of SOD was prominently decreased in the serum after cerebral ischemia in hyperlipidemia and normal groups,compared to sham operation groups and hyperlipidemia sham operation groups.SOD was prominently decreased in day 3 hyperlipidemia concurrent cerebral ischemia（P0.05）.Conclusion：Under the condition of hyperlipidemia,the inflammatory factors have not only accumulated phenomenon,but also specific expression in the difference of cerebral ischemia period,which is contributed to the recovery of the lesion in recovery phase.

关 键 词：高脂血症 脑缺血 高脂血症模型 高脂血症复合脑缺血模型 炎症反应 

分 类 号：R285.5[医药卫生—中药学]